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Relationship between volume of bathing medium and ectoenzyme activity in monolayers of cultured BPAEC

Relationship between volume of bathing medium and ectoenzyme activity in monolayers of cultured... ANDREAS PAPAPETROPOULOS, LYNN A. ELMORE, AND Vascular Biology Center and Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia Papapetropoulos, Andreas, Lynn A. Elmore, and John D. Catravas. Relatiship between volume of bathing medium and in molayers of cultured BPA. Am. J. Physiol. 271 (Lung Cell. Mol. PhysioZ. 15): L464-L469, 1996.-It is unclear whether all or a fracti of the capillary plasma volume (V,,) serves as the reacti volume (V,) for pulmary capillary endothelial s, in vivo. Cultured endothelial cell () molayers provide a cvenient model for studying -bound enzyme-V, relatiships. Bause the Michaelis-Menten parameter [maximum velocity of enzyme reacti (V,,,) = E X k,,&, where E is enzyme mass and kcat is the cstant of product formati] is inversely proportial to V,, we hypothesized that increasing the volume of medium (Vm> bathing molayers would proportially reduce the calculated V,,, (or V,,,IK,, where K, is the Michaelis cstant) values of an reacting with a substrate ly if, and as lg as, V, = V,. To test this hypothesis, studies were performed in bovine pulmary arterial grown to cfluence. Activities of angiotensincverting enzyme (ACE) and 5’-nucleotidase (NCT) were assayed in Earle’s balanced salts soluti utilizing [3H]benzoyl-Phe-Ala-Pro ( http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Lung Cellular and Molecular Physiology The American Physiological Society

Relationship between volume of bathing medium and ectoenzyme activity in monolayers of cultured BPAEC

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Publisher
The American Physiological Society
Copyright
Copyright © 1996 the American Physiological Society
ISSN
1040-0605
eISSN
1522-1504
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Abstract

ANDREAS PAPAPETROPOULOS, LYNN A. ELMORE, AND Vascular Biology Center and Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia Papapetropoulos, Andreas, Lynn A. Elmore, and John D. Catravas. Relatiship between volume of bathing medium and in molayers of cultured BPA. Am. J. Physiol. 271 (Lung Cell. Mol. PhysioZ. 15): L464-L469, 1996.-It is unclear whether all or a fracti of the capillary plasma volume (V,,) serves as the reacti volume (V,) for pulmary capillary endothelial s, in vivo. Cultured endothelial cell () molayers provide a cvenient model for studying -bound enzyme-V, relatiships. Bause the Michaelis-Menten parameter [maximum velocity of enzyme reacti (V,,,) = E X k,,&, where E is enzyme mass and kcat is the cstant of product formati] is inversely proportial to V,, we hypothesized that increasing the volume of medium (Vm> bathing molayers would proportially reduce the calculated V,,, (or V,,,IK,, where K, is the Michaelis cstant) values of an reacting with a substrate ly if, and as lg as, V, = V,. To test this hypothesis, studies were performed in bovine pulmary arterial grown to cfluence. Activities of angiotensincverting enzyme (ACE) and 5’-nucleotidase (NCT) were assayed in Earle’s balanced salts soluti utilizing [3H]benzoyl-Phe-Ala-Pro (

Journal

AJP - Lung Cellular and Molecular Physiology – The American Physiological Society

Published: Sep 1, 1996

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