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Relationship between 125IRTI-55-labeled cocaine binding sites and the serotonin transporter in rat placenta

Abstract We investigated the characteristics of cocainelike binding sites in rat placenta using 125 IRTI-55. 3 Hparoxetine binding and immunocytochemical staining for serotonin 5-hydroxytryptamine (5-HT) and for the 5-HT transporter were also used to obtain evidence for rat placental 5-HT uptake. 125 IRTI-55 saturation analyses with membranes from normal gestational day 20 placentas yielded curvilinear Scatchard plots that were resolved into high- and low-affinity components (mean dissociation constants of 0.29 and 7.9 nM, respectively). Drug competition studies with various monoamine uptake inhibitors gave rise to complex multiphasic displacement curves, although the results obtained with the selective 5-HT uptake inhibitor citalopram suggest that the 5-HT transporter is an important component of placental high-affinity 125 IRTI-55 binding. The presence of a rat placental 5-HT uptake system was additionally supported by the 3 Hparoxetine binding experiments and by the presence throughout the placenta of immunoreactivity for 5-HT and the 5-HT transporter. Immunostaining with both antibodies was most intense in the junctional zone, whereas the density of 125 IRTI-55 binding sites was greater in the placental labyrinth. This discrepancy may be due to the fact that 125 IRTI-55 appears to be labeling additional cellular components besides the 5-HT transporter. The presence of cocaine- and antidepressant-sensitive 5-HT transporters in the placenta has important implications for the possible effects of these compounds on pregnancy and fetal development. development immunocytochemistry paroxetine uptake Footnotes Address for reprint requests: J. S. Meyer, Dept. of Psychology, Tobin Hall, University of Massachusetts, Amherst, MA 01003-7710. Present address of L. P. Shearman: Laboratory of Developmental Chronobiology, Massachusetts General Hospital, 32 Fruit St., Boston, MA 02114. Copyright © 1998 the American Physiological Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

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