Regulation of spontaneous EDRF release in diabetic rat aorta by oxygen free radicals

Regulation of spontaneous EDRF release in diabetic rat aorta by oxygen free radicals M. PIEPER Department of Pharmacology Toxicology, The Medical College of Wisconsin, Milwaukee, Wisconsin 53226 Langenstroer, Peter, Galen M. Pieper. Regulation of releasein diabetic rat aorta by free . Am. J. Physiol. 263 (Heart Circ. Physiol. 32): H257-H265, 1992.-The interaction of endothelium-derived relaxing factor () -derived free may potentially play an important role in the pathophysiology of complicationsassociated with diabetes.In the present study, we investigated releasein diabetic rat aorta that is unmasked by the addition of superoxide dismutase (SOD). SOD produced a significantly greater relaxation in diabetic aorta comparedwith control aorta using both aortic ring bioassay preparations. This relaxation was unaltered by pretreatment with catalase or indomethacin. Removal of the endothelium or pretreatment with either NG-monomethyl-Larginine or methylene blue eliminated SOD-induced relaxation in both control diabetic rings. Measurement of antioxidant enzymesrevealedan elevation in catalasein diabetic aorta, with no difference in the SOD or glutathione peroxidase activity. The increasein catalaseactivity suggests increasedexposureof diabetic aorta to hydrogen peroxide. Pretreatment of rings with the catalase inhibitor, 3-amino-1,2,4-triazole, attenuated the SOD-induced relaxation in diabetic aortic rings but had no effect in control aortic rings. In summary, our observations suggestthat the diabetic rat aorta releases more than control aorta; however, the activity of on http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Heart and Circulatory Physiology The American Physiological Society

Regulation of spontaneous EDRF release in diabetic rat aorta by oxygen free radicals

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Abstract

M. PIEPER Department of Pharmacology Toxicology, The Medical College of Wisconsin, Milwaukee, Wisconsin 53226 Langenstroer, Peter, Galen M. Pieper. Regulation of releasein diabetic rat aorta by free . Am. J. Physiol. 263 (Heart Circ. Physiol. 32): H257-H265, 1992.-The interaction of endothelium-derived relaxing factor () -derived free may potentially play an important role in the pathophysiology of complicationsassociated with diabetes.In the present study, we investigated releasein diabetic rat aorta that is unmasked by the addition of superoxide dismutase (SOD). SOD produced a significantly greater relaxation in diabetic aorta comparedwith control aorta using both aortic ring bioassay preparations. This relaxation was unaltered by pretreatment with catalase or indomethacin. Removal of the endothelium or pretreatment with either NG-monomethyl-Larginine or methylene blue eliminated SOD-induced relaxation in both control diabetic rings. Measurement of antioxidant enzymesrevealedan elevation in catalasein diabetic aorta, with no difference in the SOD or glutathione peroxidase activity. The increasein catalaseactivity suggests increasedexposureof diabetic aorta to hydrogen peroxide. Pretreatment of rings with the catalase inhibitor, 3-amino-1,2,4-triazole, attenuated the SOD-induced relaxation in diabetic aortic rings but had no effect in control aortic rings. In summary, our observations suggestthat the diabetic rat aorta releases more than control aorta; however, the activity of on

Journal

AJP - Heart and Circulatory PhysiologyThe American Physiological Society

Published: Jul 1, 1992

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