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Regulation of cytosolic free Ca2+ concentration in acinar cells of rat pancreas

Regulation of cytosolic free Ca2+ concentration in acinar cells of rat pancreas Kennedyallee 70, 6000 Frankfurt (Main) 70, West Germany STREB, H., . Regulation of free Ca2+ concentration in of rat pancreas. Am. J. Physiol. 245 (Gastrointest. Liver Physiol. 8): G347-G357, 1983.-Ca2+ uptake into isolated exocrine pancreatic with highly permeable plasma membrane was determined by measuring the decrease in free Ca2’ concentration of the surrounding incubation medium with a Ca2+-specific electrode. In the presence of MgATP and respiratory substrates the free Ca2+ concentration of the incubation medium decreased rapidly after addition of leaky until a stable medium free Ca2+ concentration of 4.2 & 0.1 x lo-’ mol/l was obtained. Changes in the medium free Ca2+ concentration at steady state by addition of Ca2+ or EGTA were buffered by cellular uptake or release, respectively, until the steady-state free Ca2’ concentration was reestablished. When nonmitochondrial Ca2+ uptake was determined in the presence of a combination of mitochondrial inhibitors (10S5 mol/l antimycin, 5 x 10e6 mol/l oligomycin, and lOa mol/l azide), the rate of uptake was considerably reduced, while the steady-state concentration was unaltered. In contrast, mitochondrial uptake that could be observed in the presence of the ATPase inhibitor vanadate (2 x 10m3mol/l) proceeded at the same rate as the control, but the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Gastrointestinal and Liver Physiology The American Physiological Society

Regulation of cytosolic free Ca2+ concentration in acinar cells of rat pancreas

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Publisher
The American Physiological Society
Copyright
Copyright © 1983 the American Physiological Society
ISSN
0193-1857
eISSN
1522-1547
Publisher site
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Abstract

Kennedyallee 70, 6000 Frankfurt (Main) 70, West Germany STREB, H., . Regulation of free Ca2+ concentration in of rat pancreas. Am. J. Physiol. 245 (Gastrointest. Liver Physiol. 8): G347-G357, 1983.-Ca2+ uptake into isolated exocrine pancreatic with highly permeable plasma membrane was determined by measuring the decrease in free Ca2’ concentration of the surrounding incubation medium with a Ca2+-specific electrode. In the presence of MgATP and respiratory substrates the free Ca2+ concentration of the incubation medium decreased rapidly after addition of leaky until a stable medium free Ca2+ concentration of 4.2 & 0.1 x lo-’ mol/l was obtained. Changes in the medium free Ca2+ concentration at steady state by addition of Ca2+ or EGTA were buffered by cellular uptake or release, respectively, until the steady-state free Ca2’ concentration was reestablished. When nonmitochondrial Ca2+ uptake was determined in the presence of a combination of mitochondrial inhibitors (10S5 mol/l antimycin, 5 x 10e6 mol/l oligomycin, and lOa mol/l azide), the rate of uptake was considerably reduced, while the steady-state concentration was unaltered. In contrast, mitochondrial uptake that could be observed in the presence of the ATPase inhibitor vanadate (2 x 10m3mol/l) proceeded at the same rate as the control, but the

Journal

AJP - Gastrointestinal and Liver Physiology – The American Physiological Society

Published: Sep 1, 1983

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