Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans

Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro... Adiponectin is an adipose tissue-specific protein that is abundantly present in the circulation and suggested to be involved in insulin sensitivity and development of atherosclerosis. Because cytokines are suggested to regulate adiponectin, the aim of the present study was to investigate the interaction between adiponectin and three adipose tissue-derived cytokines (IL-6, IL-8, and TNF-α). The study was divided into three substudies as follows: 1 ) plasma adiponectin and mRNA levels in adipose tissue biopsies from obese subjects mean body mass index (BMI): 39.7 kg/m 2 , n = 6 before and after weight loss; 2 ) plasma adiponectin in obese men (mean BMI: 38.7 kg/m 2 , n = 19) compared with lean men (mean BMI: 23.4 kg/m 2 , n = 10) before and after weight loss; and 3 ) in vitro direct effects of IL-6, IL-8, and TNF-α on adiponectin mRNA levels in adipose tissue cultures. The results were that 1 ) weight loss resulted in a 51% ( P < 0.05) increase in plasma adiponectin and a 45% ( P < 0.05) increase in adipose tissue mRNA levels; 2 ) plasma adiponectin was 53% ( P < 0.01) higher in lean compared with obese men, and plasma adiponectin was inversely correlated with adiposity, insulin sensitivity, and IL-6; and 3 ) TNF-α ( P < 0.01) and IL-6 plus its soluble receptor ( P < 0.05) decreased adiponectin mRNA levels in vitro. The inverse relationship between plasma adiponectin and cytokines in vivo and the cytokine-induced reduction in adiponectin mRNA in vitro suggests that endogenous cytokines may inhibit adiponectin. This could be of importance for the association between cytokines (e.g., IL-6) and insulin resistance and atherosclerosis. interleukin-6; tumor necrosis factor-α; interleukin-8; human adipose tissue Address for reprint requests and other correspondence: J. M. Bruun, Dept. of Endocrinology and Metabolism, Aarhus Amtssygehus, Tage Hansensgade 2, DK-8000 Aarhus C, Denmark (E-mail: jmb@mail-online.dk ). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Endocrinology and Metabolism The American Physiological Society

Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans

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Publisher
The American Physiological Society
Copyright
Copyright © 2011 the American Physiological Society
ISSN
0193-1849
eISSN
1522-1555
D.O.I.
10.1152/ajpendo.00110.2003
Publisher site
See Article on Publisher Site

Abstract

Adiponectin is an adipose tissue-specific protein that is abundantly present in the circulation and suggested to be involved in insulin sensitivity and development of atherosclerosis. Because cytokines are suggested to regulate adiponectin, the aim of the present study was to investigate the interaction between adiponectin and three adipose tissue-derived cytokines (IL-6, IL-8, and TNF-α). The study was divided into three substudies as follows: 1 ) plasma adiponectin and mRNA levels in adipose tissue biopsies from obese subjects mean body mass index (BMI): 39.7 kg/m 2 , n = 6 before and after weight loss; 2 ) plasma adiponectin in obese men (mean BMI: 38.7 kg/m 2 , n = 19) compared with lean men (mean BMI: 23.4 kg/m 2 , n = 10) before and after weight loss; and 3 ) in vitro direct effects of IL-6, IL-8, and TNF-α on adiponectin mRNA levels in adipose tissue cultures. The results were that 1 ) weight loss resulted in a 51% ( P < 0.05) increase in plasma adiponectin and a 45% ( P < 0.05) increase in adipose tissue mRNA levels; 2 ) plasma adiponectin was 53% ( P < 0.01) higher in lean compared with obese men, and plasma adiponectin was inversely correlated with adiposity, insulin sensitivity, and IL-6; and 3 ) TNF-α ( P < 0.01) and IL-6 plus its soluble receptor ( P < 0.05) decreased adiponectin mRNA levels in vitro. The inverse relationship between plasma adiponectin and cytokines in vivo and the cytokine-induced reduction in adiponectin mRNA in vitro suggests that endogenous cytokines may inhibit adiponectin. This could be of importance for the association between cytokines (e.g., IL-6) and insulin resistance and atherosclerosis. interleukin-6; tumor necrosis factor-α; interleukin-8; human adipose tissue Address for reprint requests and other correspondence: J. M. Bruun, Dept. of Endocrinology and Metabolism, Aarhus Amtssygehus, Tage Hansensgade 2, DK-8000 Aarhus C, Denmark (E-mail: jmb@mail-online.dk ).

Journal

AJP - Endocrinology and MetabolismThe American Physiological Society

Published: Sep 1, 2003

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