DAVID BORSOOK AND STEVEN E. HYIQAN Laboratory of Molecular and Developmental Neuroscience, Departments Neurology, and Psychiatry, Harvard Medical School and Massachusetts Boston, Massachusetts 02114 Borsook, David, and Steven E. Hyman. gene regulation in the neuroendocrine hypothalamus: a model of gene regulation in the CNS. Am. J. Physiol. 269 (Endocrinol. Metab. 32): E393-E408, 1995.-During the past decade, a great deal of progress has been made in studying the mechanisms by which transcription of neuropeptides is regulated by second messengers and neural activity. Such investigations, which have depended to a great extent on the use of transformed cell lines, are far from complete. Yet a major challenge for the coming decade is to understand the regulation of neuropeptide genes by physiologically and pharmacologically relevant stimuli in appropriate cell types in vivo. The gene, a member of the opioid gene family, has served as a model to study regulated transcription, not only in cell lines, but also in central (e.g., hypothalamic) and peripheral (e.g., adrenal) neuroendocrine tissues. Here we review regulation of gene expression in the hypothalamus. Several approaches, including in situ hybridization, use of transgenic mice, and the adaptation of electrophoretic mobility shift assays to complex tissues, have played critical
AJP - Endocrinology and Metabolism – The American Physiological Society
Published: Sep 1, 1995
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