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Castillo, L., M. SQnchez, J. Vogt, T. E. Chapman, T. C. DeRojas-Walker, S. R. Tannenbaum, A. M. Ajami, V. R. Young. Plasma , citrulline, ornithine kinetics in adults, with observations on . Am. J. Physiol. 268 (Endocrinol. Metab. 31): E360-E367, 1995.-The plasma fluxes of ornithine (Orn), (Arg), citrulline (Cit) rate of conversion of labeled ornithineto-citrulline (Qorn + tit) were estimated in six healthy adult men receiving an -rich or -free L-amino acidbased diet, each for 6 days. On day 7 an 8-h (3-h fast, 5-h fed) primed continuous intravenous infusion of L-[guanido15N,15N] , L-[ureido- 13C]citrulline, L-[5,5,2H2]ornithine, L-[5,5,5-2H3]leucine was conducted. Mean citrulline fluxes (pmol. kg-l. h-l) were 10.4-13.6 for the various conditions /or diets remained unchanged (P > 0.05). flux was lowered (P < 0.01) by 38% for fed state during -free period. Ornithine fluxes for -rich were (P < 0.01) reduced with the -free diet. Rates of Qorn+cit declined by 30% (P < .05) during the fed -free period. Short-term restriction in the dietary supply of did not alter the rate of whole body . One subject showed a very high output of nitrate on -free diet (6 times average for remaining subjects). nitrate; ity 15N-labeling; flux; homeostasis;
AJP - Endocrinology and Metabolism – The American Physiological Society
Published: Feb 1, 1995
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