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Phosphatidylcholine-directed phospholipase C: activation by complement C5b-9

Phosphatidylcholine-directed phospholipase C: activation by complement C5b-9 ANDREY V. CYBULSKY AND MARIE-DANIELLE CYR Department of Medicine, Royal Victoria Hospital, McGill University, Montreal, Quebec H3A lA1, Canada Cybulsky, Andrey V., and Marie-Danielle Cyr. Phosphatidylcholine-directed C: activation by complement.Am. J. Physiol. 265 (Renal Fhid EZectroZyte Physiol. 34): F551-F560, 1993.-In rat membranousnephropathy, complementinducesglomerularepithelial cell (GEC) injury and proteinuria. In cultured rat GEC, stimulates a phosphoinositide-directed(PL) C and products of PLC downregulate C5b-g-mediated GEC injury. We now report that CSb-g-induced hydrolysis of phosphatidylcholine (PC) provides an additional sourceof 1,2-diacylglycerol (DAG). PC was labeled in intact GEC by brief incubation with l-O[alizyl-3H]2-lyso-PC. Assembly of stimulated an increase in PC-derived [3H]DAG (173 & 18% control), which was reducedin GEC depletedof protein kinaseC (PKC) by prolonged preincubation with phorbol 12-myristate 13-acetate (PMA). Similar to , [“H]DAG was releasedfrom PC after brief incubation of GEC with Ca2+ ionophore A23187 plus PMA. The increases [3H]DAG induced by and A23187 plus in PMA were paralleled by increasesin DAG mass. also increased[3H]phosphatidic acid (PA; 182 t 37% control), but there wasno significant interconversion of DAG and PA. Thus DAG probably originated via PLC. PC-directed PLC activity was also studied in GEC homogenates releaseof [14C]DAG by from exogenous1-palmitoyl-2- [ arachidonoyl- 14C] PC. PLC activity was present at http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Renal Physiology The American Physiological Society

Phosphatidylcholine-directed phospholipase C: activation by complement C5b-9

A. V. Cybulsky and M. D. Cyr
AJP - Renal Physiology , Volume 265: F551 – Oct 1, 1993
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Publisher
The American Physiological Society
Copyright
Copyright © 1993 the American Physiological Society
ISSN
0363-6127
eISSN
1522-1466
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Abstract

ANDREY V. CYBULSKY AND MARIE-DANIELLE CYR Department of Medicine, Royal Victoria Hospital, McGill University, Montreal, Quebec H3A lA1, Canada Cybulsky, Andrey V., and Marie-Danielle Cyr. Phosphatidylcholine-directed C: activation by complement.Am. J. Physiol. 265 (Renal Fhid EZectroZyte Physiol. 34): F551-F560, 1993.-In rat membranousnephropathy, complementinducesglomerularepithelial cell (GEC) injury and proteinuria. In cultured rat GEC, stimulates a phosphoinositide-directed(PL) C and products of PLC downregulate C5b-g-mediated GEC injury. We now report that CSb-g-induced hydrolysis of phosphatidylcholine (PC) provides an additional sourceof 1,2-diacylglycerol (DAG). PC was labeled in intact GEC by brief incubation with l-O[alizyl-3H]2-lyso-PC. Assembly of stimulated an increase in PC-derived [3H]DAG (173 & 18% control), which was reducedin GEC depletedof protein kinaseC (PKC) by prolonged preincubation with phorbol 12-myristate 13-acetate (PMA). Similar to , [“H]DAG was releasedfrom PC after brief incubation of GEC with Ca2+ ionophore A23187 plus PMA. The increases [3H]DAG induced by and A23187 plus in PMA were paralleled by increasesin DAG mass. also increased[3H]phosphatidic acid (PA; 182 t 37% control), but there wasno significant interconversion of DAG and PA. Thus DAG probably originated via PLC. PC-directed PLC activity was also studied in GEC homogenates releaseof [14C]DAG by from exogenous1-palmitoyl-2- [ arachidonoyl- 14C] PC. PLC activity was present at

Journal

AJP - Renal Physiology – The American Physiological Society

Published: Oct 1, 1993

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