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Osmoregulation of Na(+)-inositol cotransporter activity and mRNA levels in brain glial cells

STRANGE Division of Nephrology, Department of Medicine and Department of Anesthesiology, Children’s Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Renal Division, Department of Medicine, Johns Hopkins University, Baltimore, Maryland 21205 Paredes, Ana, Michael McManus, H. Moo Kwon, and Kevin Strange. er activity and mRNA levels in brain glial . Am. J. Physiol. 263 (CeU Physiol. 32): C1282-Cl288, 1992.-During plasma hypertonicity brain volume is regulated acutely by electrolyte uptake and chronically by accumulation of organic solutes such as inositol. Cultured rat C6 glioma , an astrocyte-like cell line, show a similar pattern of volume control. Volume regulatory accumulation of inositol requires external inositol, indicating that membrane transport plays a central role in this process. The inositol uptake pathway is Na+ dependent and exhibits Michaelis-Menten kinetics. Chronic hypertonic acclimation results in a twofold increase in the maximum velocity of the transporter without changing the K,. Hypertonic stress also results in a 17-fold increase in transporter mRNA. Elevation of mRNA levels precedes activation of the transporter by 4-6 h, suggesting that increased inositol uptake is mediated by synthesis and membrane insertion of new transport proteins. Reacclimation of hypertonic to isotonicity causes a rapid reduction of transporter mRNA levels to control http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

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