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Maffia, M., R. Acierno, E. Cillo, and C. Storelli. Naâ-Dglucose cotransport by intestinal BBMVs of the Antarctic IFernatonzus hernacchii. Am. J. PhysioZ. 271 (Regulatory Ir2tegrative Comp. PhysioZ. 40): Rl576-R1583, 1996.Intestinal nutrient in adapted to low temperature was investigated by isolating, with a Mg2+-precipitation procedure, brush-border membrane vesicles (BBMVs) from s of the Antarctic teleost Tkematomus bernacchii. In particular, transport was analyzed by measuring both 1) fluorescence changes of the electrical potentialsensitive dye 3,3â-diethylthiadicarbocyanine iodide [DiSC2(5)] and 2) intravesicular uptake of D-[â4C]glucoSe. Results demonstrated that transport of across intestinal BBMs of the Antarctic is stimulated by the presence of a transmembrane Na + gradient (out > in) and was specifically inhibited by phloridzin. Furthermore, Na+-dependent uptake was strongly enhanced by the presence of an electrical potential (inside-negative) across the membrane. There was a marked difference in temperature dependence of Nat -sugar cotransport between the Antarctic and a temperate , such as the European yellow eel. Na+dependent uptake in T bernacchii intestinal BBMV reached its maximal rate at -2-0°C (close to living temperature) and was exponentially inactivated by incubation at higher temperatures. Kinetic analysis of D-glUCcWe influx indicated the presence of a single Na+-dependent carrier process (apparent maximal carrier-mediated influx
AJP - Regulatory, Integrative and Comparative Physiology – The American Physiological Society
Published: Dec 1, 1996
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