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Myofibrillar end of the creatine phosphate energy shuttle

F., P. J. AND S. P. BESSMAN. Myofibrillar Reagents. All reagents were of the highest purity obtainable. phosphate (CP), ADP, ATP, P1,P5diadenosine 5’-pentaphosphate (AP5A), , and 2,4-dinitrofluorobenzene (DNFB) were purchased from Sigma Chemical. Alamine 336 is a product of General Mills Chemicals. Freon TF was obtained from MillerStephenson Chemical. Preparation of glycerinated musclefibers. Fiber bundles of 2 to 3 mm diam and -4.5 cm in length from the psoas muscle of male white albino rabbits were tied at slightly stretch state to a glass slide and immersed at 5°C in 50% glycerol storage solution according to the method of Hanson and Huxley (12) with a slight modification in which 10 mM maleate-potassium buffer, pH 7.0, was role ; force of contraction; com- used instead of 6.7 mM phosphate buffer. After 24 h at partmentation; glycerinated fibers; striated muscle 5°C the fibers were immersed in fresh glycerol storage solution and stored at -2OOC. General condition and protocol for recording contraction THE AVAILABLE KINETICS and biochemical evidence (27, and relaxation. Fibers were taken from the storage solution, drained, and placed in -50 ml of fresh storage 28,36) have indicated the possibility of a close functional solution. Fiber bundles were teased http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

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