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Membrane leakage and increased content of Na+-K+ pumps and Ca2+ in human muscle after a 100-km run

Membrane leakage and increased content of Na+-K+ pumps and Ca2+ in human muscle after a 100-km run Abstract During prolonged exercise, changes in the ionic milieu in and surrounding the muscle fibers may lead to fatigue or damage of the muscle and thereby impair performance. In 10 male subjects, we investigated the effects of 100 km running on muscle and plasma electrolyte contents, muscle Na + -K + pump content, and plasma concentrations of creatine kinase (CK) and lactate dehydrogenase (LDH). After completion of a 100-km run, significant increases were found in plasma K + (from 4.0 ± 0.1 to 5.5 ± 0.2 mM, P < 0.001), muscle Na + -K + pump content (from 334 ± 11 to 378 ± 17 pmol/g, P < 0.05), and total muscle Ca 2+ content (from 0.84 ± 0.03 to 1.02 ± 0.04 μmol/g, P < 0.001). There was also a large increase in the plasma levels of the muscle-specific enzymes CK and LDH, which reached peak values at the end of the run and lasted several days after the run, indicating that a significant degree of muscle membrane leakage was present. The simultaneous occurrence of raised cellular Ca 2+ content and muscle membrane leakage supports the theory that Ca 2+ plays a role in the initiation of degenerative processes in muscles after severe exercise. muscle enzymes potassium balance endurance running Footnotes The study was supported by a grant from the Danish Biomembrane Center. Address for reprint requests and other correspondence: K. Overgaard, Dept. of Sport Science, Univ. of Aarhus, Katrinebjergvej 89C, DK-8200 Århus N, Denmark (E-mail: ko@fi.au.dk ). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “ advertisement ” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. First published January 4, 2002;10.1152/japplphysiol.00669.2001 Copyright © 2002 the American Physiological Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Physiology The American Physiological Society

Membrane leakage and increased content of Na+-K+ pumps and Ca2+ in human muscle after a 100-km run

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Publisher
The American Physiological Society
Copyright
Copyright © 2011 the American Physiological Society
ISSN
8750-7587
eISSN
1522-1601
DOI
10.1152/japplphysiol.00669.2001
pmid
11960939
Publisher site
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Abstract

Abstract During prolonged exercise, changes in the ionic milieu in and surrounding the muscle fibers may lead to fatigue or damage of the muscle and thereby impair performance. In 10 male subjects, we investigated the effects of 100 km running on muscle and plasma electrolyte contents, muscle Na + -K + pump content, and plasma concentrations of creatine kinase (CK) and lactate dehydrogenase (LDH). After completion of a 100-km run, significant increases were found in plasma K + (from 4.0 ± 0.1 to 5.5 ± 0.2 mM, P < 0.001), muscle Na + -K + pump content (from 334 ± 11 to 378 ± 17 pmol/g, P < 0.05), and total muscle Ca 2+ content (from 0.84 ± 0.03 to 1.02 ± 0.04 μmol/g, P < 0.001). There was also a large increase in the plasma levels of the muscle-specific enzymes CK and LDH, which reached peak values at the end of the run and lasted several days after the run, indicating that a significant degree of muscle membrane leakage was present. The simultaneous occurrence of raised cellular Ca 2+ content and muscle membrane leakage supports the theory that Ca 2+ plays a role in the initiation of degenerative processes in muscles after severe exercise. muscle enzymes potassium balance endurance running Footnotes The study was supported by a grant from the Danish Biomembrane Center. Address for reprint requests and other correspondence: K. Overgaard, Dept. of Sport Science, Univ. of Aarhus, Katrinebjergvej 89C, DK-8200 Århus N, Denmark (E-mail: ko@fi.au.dk ). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “ advertisement ” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. First published January 4, 2002;10.1152/japplphysiol.00669.2001 Copyright © 2002 the American Physiological Society

Journal

Journal of Applied Physiology – The American Physiological Society

Published: May 1, 2002

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