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University BARNARD, of California, AND hs GLEN Department Angeles, California K. GRIMDITCH 90024 Materials. The materials used for the isolation procedure were the same as described previously (11). The purified porcine insulin was donated by Eli Lilly (Indianapolis, IN). Cytochalasin B was obtained from Aldrich Chemical. The tracers, D- [3H], L- [ 14C], D[Wlmannitol, and [3H]cytochalasin B with specific activities of 33.1 Ci/mol, 47.0 mCi/mmol, 48.7 mCi/mmol, and 18.5 Ci/mmol, respectively, were purchased from kinetics; cytochalasin B; sarcolemmal New England Nuclear (Boston, MA). All other chemicals were obtained from Sigma Chemical. Animals. Female Sprague-Dawley rats were purchased SKELETAL MUSCLE is a major tissue for blood from Charles River Laboratories (Wilmington, MA) and utilization and a primary target tissue for insulin action. weighed -250 g at the time of death. Standard rat chow The mechanism by which insulin acts to increase and water were provided ad libitum to all rats. Unless in this important tissue is unknown. Several otherwise stated, n refers to the number of SL preparamodels have been used to examine the mechanism of tions per group. insulin action. To date, studies examining uptake SL isolation. Skeletal muscle SL were prepared in skeletal muscle have primarily used
AJP - Endocrinology and Metabolism – The American Physiological Society
Published: May 1, 1988
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