MATERIALS METHODS As a rule, the experiments were performed on retinas of domestic chicks. A few experiments were carried out on retinas of adult domestic hens pigeons, as well as of toads (Bufo) lizards (Tupinambis). The chicks were killed by capitation. The eyeballs were removed, sectioned along the equator, the internal hemispheres, their vitreous humor having been drawn off, were at once placed in a bath of Ringer solution. Observations were ma on such internal hemisphere preparations or, more often, on fragments of retina tached from them. In part of these, the pigment epithelium was subsequently dissected away so that preparations consisting only of the nervous portion of the retina were obtained, which could be mounted on a flat surface with either the inner or the outer surface facing upward. The preparations were maintained wholly immersed in Ringer solution at room temperature (22-26OC.). The composition of the Ringer solution which, in these experiments, was employed as a âstard,â was as follows (mM): NaCl 100.0, KC1 2.0, CaCl2 0.5, MgS04 0.5, NaHC03 30.0, NaH2P04 1.0, glucose 20.0. 1 This investigation was supported by Research Grant B-02219 Institute of Neurological Diseases Blindness, Bethesda, Md. 2 Fellow of the Conselho National
Journal of Neurophysiology – The American Physiological Society
Published: Jul 1, 1966
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