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Keratinocyte growth factor stimulates CFTR-independent fluid secretion in the fetal lung in vitro

Keratinocyte growth factor stimulates CFTR-independent fluid secretion in the fetal lung in vitro MXTERIALS AND METHODS EPITHELIAL airways and the alveoli of the actively secrete (l&13,16, 27, 32). The lumen-negative transepithelial potential difference (PD) and the reduction of transepithelial PD in cultured explants of trachea and by the Cltransport inhibitor bumetanide support the concept that production is coupled to active Clsecretion (9,12,13). morphogenesis is intimately linked with secretion in . Overdistension of the by tracheal ligation or obstruction of the trachea or bronchus accelerates normal growth and morphogenesis (1, 25). In contrast, excessive loss of the by chronic tracheal drainage retards growth and morphogenesis (1,31). Recently, keratinocyte growth factor () was found to cause pulmonary cystadenomas in transgenic mice in which was expressed in respiratory epithelial cells (26). This suggests that plays a role in the 1040-0605/96 $5.00 Copyright o 1996 CELLS of the conducting expZant culture. buds were isolated from mouse embryos at 12-13 days post-coitus (PC) obtained from the breeding of FVB/N mice or of CFTR +/- mice (kindly provided by Dr. Beverly Koller, Univ. of North Carolina). The morning of the day of vaginal plug was defined as day 0 pc. buds were cultured on 25mm diameter and 0.45urn pore size Cyclopore membranes (Falcon 3090) inserted into six-well http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Lung Cellular and Molecular Physiology The American Physiological Society

Keratinocyte growth factor stimulates CFTR-independent fluid secretion in the fetal lung in vitro

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Publisher
The American Physiological Society
Copyright
Copyright © 1996 the American Physiological Society
ISSN
1040-0605
eISSN
1522-1504
Publisher site
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Abstract

MXTERIALS AND METHODS EPITHELIAL airways and the alveoli of the actively secrete (l&13,16, 27, 32). The lumen-negative transepithelial potential difference (PD) and the reduction of transepithelial PD in cultured explants of trachea and by the Cltransport inhibitor bumetanide support the concept that production is coupled to active Clsecretion (9,12,13). morphogenesis is intimately linked with secretion in . Overdistension of the by tracheal ligation or obstruction of the trachea or bronchus accelerates normal growth and morphogenesis (1, 25). In contrast, excessive loss of the by chronic tracheal drainage retards growth and morphogenesis (1,31). Recently, keratinocyte growth factor () was found to cause pulmonary cystadenomas in transgenic mice in which was expressed in respiratory epithelial cells (26). This suggests that plays a role in the 1040-0605/96 $5.00 Copyright o 1996 CELLS of the conducting expZant culture. buds were isolated from mouse embryos at 12-13 days post-coitus (PC) obtained from the breeding of FVB/N mice or of CFTR +/- mice (kindly provided by Dr. Beverly Koller, Univ. of North Carolina). The morning of the day of vaginal plug was defined as day 0 pc. buds were cultured on 25mm diameter and 0.45urn pore size Cyclopore membranes (Falcon 3090) inserted into six-well

Journal

AJP - Lung Cellular and Molecular Physiology – The American Physiological Society

Published: Dec 1, 1996

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