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Intracellular oxygen supply during hypoxia

Intracellular oxygen supply during hypoxia of Medicine, Atlanta, Georgia 30322 DEAN P. JONES FRANCES Department of Biochemistry, Emory G. KENNEDY University School JONES,DEAN P., FRANCES G.KENNEDY. IntraceZZuZar purchased from Sigma Chemical. FCCP (carbonylcya SUJ@” . Am. J. Physiol. 243 (Cell Physnide-p-trifluoromethoxyphenylhydrazone) was puriol. 12): C247-C253, 1982.-The of O2 to chased from Boehringer Mannheim GmbH, Mannheim, mitochondria was studied in single-cell suspensions of rat heWest Germany. Other chemicals were reagent grade patocytes by measuring the 02 dependence of oxidation of purchased locally. Deionized water was used throughout. cytochromes. Values were obtained by adding stardized 02’ Cell preparation incubations. Hepatocytes were containing solutions to anaerobic cell suspensions observing isolated from male white rats (Charles River, 180-250 g) absorbance changes at wavelength pairs specific for cytofed ad libitum as previously described (22). These cells chromes a + a3,c + cl, b 561 + b566. Half-maximal oxidation, calculated relative to aerobic cells, occurred at 3.5, 6.2, 4.9 are metabolically intact as judged by many criteria, e.g., respiratory rate (13), adenylate pools (13), pyridine nuPM 02, respectively. These values are similar to those for changes in cellular ATP/ADP, lactate/pyruvate, NADH/ cleotide pools (ll), cytochromes content (15), mixedNAD’ but are much higher than corresponding values for function oxidation rates http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

Intracellular oxygen supply during hypoxia

D. P. Jones and F. G. Kennedy
AJP - Cell Physiology , Volume 243: C247 – Nov 1, 1982
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Publisher
The American Physiological Society
Copyright
Copyright © 1982 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
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Abstract

of Medicine, Atlanta, Georgia 30322 DEAN P. JONES FRANCES Department of Biochemistry, Emory G. KENNEDY University School JONES,DEAN P., FRANCES G.KENNEDY. IntraceZZuZar purchased from Sigma Chemical. FCCP (carbonylcya SUJ@” . Am. J. Physiol. 243 (Cell Physnide-p-trifluoromethoxyphenylhydrazone) was puriol. 12): C247-C253, 1982.-The of O2 to chased from Boehringer Mannheim GmbH, Mannheim, mitochondria was studied in single-cell suspensions of rat heWest Germany. Other chemicals were reagent grade patocytes by measuring the 02 dependence of oxidation of purchased locally. Deionized water was used throughout. cytochromes. Values were obtained by adding stardized 02’ Cell preparation incubations. Hepatocytes were containing solutions to anaerobic cell suspensions observing isolated from male white rats (Charles River, 180-250 g) absorbance changes at wavelength pairs specific for cytofed ad libitum as previously described (22). These cells chromes a + a3,c + cl, b 561 + b566. Half-maximal oxidation, calculated relative to aerobic cells, occurred at 3.5, 6.2, 4.9 are metabolically intact as judged by many criteria, e.g., respiratory rate (13), adenylate pools (13), pyridine nuPM 02, respectively. These values are similar to those for changes in cellular ATP/ADP, lactate/pyruvate, NADH/ cleotide pools (ll), cytochromes content (15), mixedNAD’ but are much higher than corresponding values for function oxidation rates

Journal

AJP - Cell Physiology – The American Physiological Society

Published: Nov 1, 1982

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