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Intestinal absorption and metabolism of ascorbic acid in rainbow trout

Intestinal absorption and metabolism of ascorbic acid in rainbow trout RICHARD C. ROSE AND JEE-LIM CHOI Department Physiology, University North Dakota School Medicine, Grand Forks, North Dakota 58202; and Intercollege Nutrition Graduate Program, Pennsylvania State University, University Park, Pennsylvania 16802 ROSE, RICHARD C., AND JEE-LIM CHOI. IntestinaZ absorption and metabolism in rainbow trout. Am. J. Physiol. 258 (Regulatory Integrative Comp. Physiol. 27): R1238-Rl241, 1990.-Transport and metabolism the reduced and oxidized forms vitamin C were evaluated in trout intestine in vitro. Transepithelial fluxes [ 14C] were determined with the substrate present at 10 PM. The mucosa-to-serosa flux was fourfold greater than the serosa-to-mucosa flux with present in both bathing solutions. Cellular accumulation the reduced molecule occurred with a final tissue-tomedium ratio 1.64. influx across the brushborder membrane was determined under conditions that inhibit transport other nutrients. Influx was not reduced because the presence the D- or L-stereoisomeric form glucose but was highly dependent on the presence Na in the bathing medium. Brush-border influx was saturable with a K, 220 ,uM. Short-term (8 min) exposure loops to the oxidized form vitamin C, dehydro-L- , was followed by high-performance liquid chromatography analysis the 14C label present in the tissue. Total uptake substrate was greater from the serosal solution than from the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Regulatory, Integrative and Comparative Physiology The American Physiological Society

Intestinal absorption and metabolism of ascorbic acid in rainbow trout

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Publisher
The American Physiological Society
Copyright
Copyright © 1990 the American Physiological Society
ISSN
0363-6119
eISSN
1522-1490
Publisher site
See Article on Publisher Site

Abstract

RICHARD C. ROSE AND JEE-LIM CHOI Department Physiology, University North Dakota School Medicine, Grand Forks, North Dakota 58202; and Intercollege Nutrition Graduate Program, Pennsylvania State University, University Park, Pennsylvania 16802 ROSE, RICHARD C., AND JEE-LIM CHOI. IntestinaZ absorption and metabolism in rainbow trout. Am. J. Physiol. 258 (Regulatory Integrative Comp. Physiol. 27): R1238-Rl241, 1990.-Transport and metabolism the reduced and oxidized forms vitamin C were evaluated in trout intestine in vitro. Transepithelial fluxes [ 14C] were determined with the substrate present at 10 PM. The mucosa-to-serosa flux was fourfold greater than the serosa-to-mucosa flux with present in both bathing solutions. Cellular accumulation the reduced molecule occurred with a final tissue-tomedium ratio 1.64. influx across the brushborder membrane was determined under conditions that inhibit transport other nutrients. Influx was not reduced because the presence the D- or L-stereoisomeric form glucose but was highly dependent on the presence Na in the bathing medium. Brush-border influx was saturable with a K, 220 ,uM. Short-term (8 min) exposure loops to the oxidized form vitamin C, dehydro-L- , was followed by high-performance liquid chromatography analysis the 14C label present in the tissue. Total uptake substrate was greater from the serosal solution than from the

Journal

AJP - Regulatory, Integrative and Comparative PhysiologyThe American Physiological Society

Published: May 1, 1990

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