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Insulinotropic action of glutamic acid dimethyl ester

Insulinotropic action of glutamic acid dimethyl ester ester ABDULLAH SENER, IGNACIO CONGET, JOANNE RASSCHAERT, VMANE LECLERCQ-MEYER, MARIA L. VILLANUEVA-PENACARRILLO, ISABEL VALVERDE, AND WILLY J. MALAISSE Laboratory of Experimental Medicine, Brussels Free University, B-l 070 Brussels, and Fundacih Jimenez Dfaz, 28040 Madrid, Spain Sener, Abdullah, Ignacio Conget, Joanne Rasschaert, Viviane Leclercq-Meyer, Maria L. Villanueva-Pefiacarrillo, Isabel Valverde, and Willy J. Malaisse. Insulinotropit action ester. Am. J. Physiol. 267 (EndocrinoZ. Metab. 30): E573-E584, 1994.-Glutamic ester (GME; 3.0-10.0 mM) enhanced insulin release evoked by 6.0-8.3 mM D-glucose, 1.0-10.0 mM L-leucine, or 5.0-10.0 mM 2-amino-bicyclo(2,2,1)heptane-2-carboxylic , causing a shift to the left of the sigmoidal relationship between insulin output and D-glucose concentration. In the absence of D-glucose, GME also unmasked the insulinotropic potential of glibenclamide. In islets exposed to L-leucine, the insulinotropic action of GME coincided with an early fall and later increase in 86Rb outflow and augmentation of 45Ca outflow from prelabeled islets. The measurement of O2 uptake, NH,+ output, production of 14C02 from islets prelabeled with [U-14C]palmitate, generation of 14C-labeled amino s and 14C02 from the ester of either L-[lJ4C]glutamic or L-[U14C]glutamic , and D-[2-‘4C]glucose as well as D-[614C]glucose oxidation in the presence or absence of GME indicated that the latter ester was efficiently converted to L-glutamate and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Endocrinology and Metabolism The American Physiological Society

Insulinotropic action of glutamic acid dimethyl ester

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Publisher
The American Physiological Society
Copyright
Copyright © 1994 the American Physiological Society
ISSN
0193-1849
eISSN
1522-1555
Publisher site
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Abstract

ester ABDULLAH SENER, IGNACIO CONGET, JOANNE RASSCHAERT, VMANE LECLERCQ-MEYER, MARIA L. VILLANUEVA-PENACARRILLO, ISABEL VALVERDE, AND WILLY J. MALAISSE Laboratory of Experimental Medicine, Brussels Free University, B-l 070 Brussels, and Fundacih Jimenez Dfaz, 28040 Madrid, Spain Sener, Abdullah, Ignacio Conget, Joanne Rasschaert, Viviane Leclercq-Meyer, Maria L. Villanueva-Pefiacarrillo, Isabel Valverde, and Willy J. Malaisse. Insulinotropit action ester. Am. J. Physiol. 267 (EndocrinoZ. Metab. 30): E573-E584, 1994.-Glutamic ester (GME; 3.0-10.0 mM) enhanced insulin release evoked by 6.0-8.3 mM D-glucose, 1.0-10.0 mM L-leucine, or 5.0-10.0 mM 2-amino-bicyclo(2,2,1)heptane-2-carboxylic , causing a shift to the left of the sigmoidal relationship between insulin output and D-glucose concentration. In the absence of D-glucose, GME also unmasked the insulinotropic potential of glibenclamide. In islets exposed to L-leucine, the insulinotropic action of GME coincided with an early fall and later increase in 86Rb outflow and augmentation of 45Ca outflow from prelabeled islets. The measurement of O2 uptake, NH,+ output, production of 14C02 from islets prelabeled with [U-14C]palmitate, generation of 14C-labeled amino s and 14C02 from the ester of either L-[lJ4C]glutamic or L-[U14C]glutamic , and D-[2-‘4C]glucose as well as D-[614C]glucose oxidation in the presence or absence of GME indicated that the latter ester was efficiently converted to L-glutamate and

Journal

AJP - Endocrinology and Metabolism – The American Physiological Society

Published: Oct 1, 1994

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