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B. Lambrecht (2006)
Alveolar macrophage in the driver's seat.Immunity, 24 4
K. Ozaki, W. Leonard (2002)
Cytokine and Cytokine Receptor Pleiotropy and Redundancy*The Journal of Biological Chemistry, 277
P. Barnes (2008)
Immunology of asthma and chronic obstructive pulmonary diseaseNature Reviews Immunology, 8
M. Epstein (2004)
Do Mouse Models of Allergic Asthma Mimic Clinical Disease?International Archives of Allergy and Immunology, 133
T. Brock, R. McNish, M. Peters-Golden (1999)
Arachidonic Acid Is Preferentially Metabolized by Cyclooxygenase-2 to Prostacyclin and Prostaglandin E2 *The Journal of Biological Chemistry, 274
M. Schuyler, K. Gott, P. Haley (1991)
Experimental murine hypersensitivity pneumonitis.Cellular immunology, 136 2
C. Lemière, P. Ernst, R. Olivenstein, Y. Yamauchi, K. Govindaraju, M. Ludwig, James Martin, Q. Hamid (2006)
Airway inflammation assessed by invasive and noninvasive means in severe asthma: eosinophilic and noneosinophilic phenotypes.The Journal of allergy and clinical immunology, 118 5
P. Barnes (2000)
Endogenous inhibitory mechanisms in asthma.American journal of respiratory and critical care medicine, 161 3 Pt 2
S. Radhakrishnan, K. Iijima, Takao Kobayashi, H. Kita, L. Pease (2005)
Dendritic cells activated by cross-linking B7-DC (PD-L2) block inflammatory airway disease.The Journal of allergy and clinical immunology, 116 3
M. Murakami, T. Kambe, S. Shimbara, I. Kudo (1999)
Functional Coupling Between Various Phospholipase A2s and Cyclooxygenases in Immediate and Delayed Prostanoid Biosynthetic Pathways*The Journal of Biological Chemistry, 274
M. Cundall, Yongchang Sun, C. Miranda, J. Trudeau, Steve Barnes, S. Wenzel (2003)
Neutrophil-derived matrix metalloproteinase-9 is increased in severe asthma and poorly inhibited by glucocorticoids.The Journal of allergy and clinical immunology, 112 6
A. Mantovani, A. Sica, S. Sozzani, P. Allavena, A. Vecchi, M. Locati (2004)
The chemokine system in diverse forms of macrophage activation and polarization.Trends in immunology, 25 12
B. Levy, C. Bonnans, E. Silverman, L. Palmer, G. Marigowda, E. Israel (2005)
Diminished lipoxin biosynthesis in severe asthma.American journal of respiratory and critical care medicine, 172 7
J. Atkinson, R. Senior (2003)
Matrix metalloproteinase-9 in lung remodeling.American journal of respiratory cell and molecular biology, 28 1
Wisam Al-Ramli, D. Préfontaine, F. Chouiali, James Martin, R. Olivenstein, C. Lemière, Q. Hamid (2009)
T(H)17-associated cytokines (IL-17A and IL-17F) in severe asthma.The Journal of allergy and clinical immunology, 123 5
Hydar Ali, R. Panettieri (2005)
Anaphylatoxin C3a receptors in asthmaRespiratory Research, 6
Isabelle Tillie-Leblond, P. Gosset, Andréa‐Bernard Tonnel (2005)
Inflammatory events in severe acute asthmaAllergy, 60
R. Warner, N. Lukacs, S. Shapiro, Narasimharao Bhagarvathula, Kamalakar Nerusu, J. Varani, Kent Johnson (2004)
Role of metalloelastase in a model of allergic lung responses induced by cockroach allergen.The American journal of pathology, 165 6
C. Vancheri, C. Mastruzzo, M. Sortino, N. Crimi (2004)
The lung as a privileged site for the beneficial actions of PGE2.Trends in immunology, 25 1
C. Ordoñez, T. Shaughnessy, M. Matthay, J. Fahy (2000)
Increased neutrophil numbers and IL-8 levels in airway secretions in acute severe asthma: Clinical and biologic significance.American journal of respiratory and critical care medicine, 161 4 Pt 1
Bei-bei Jin, Wen-bing Xu (2012)
Hypersensitivity pneumonitisChinese Journal of Asthma, 32
M. Peters-Golden (2004)
The alveolar macrophage: the forgotten cell in asthma.American journal of respiratory cell and molecular biology, 31 1
K. Nagao, Hiroyuki Tanaka, M. Komai, T. Masuda, S. Narumiya, H. Nagai (2003)
Role of prostaglandin I2 in airway remodeling induced by repeated allergen challenge in mice.American journal of respiratory cell and molecular biology, 29 3 Pt 1
P. Bogaert, K. Tournoy, T. Naessens, J. Grooten (2009)
Where asthma and hypersensitivity pneumonitis meet and differ: noneosinophilic severe asthma.The American journal of pathology, 174 1
D. Huang, Brad Sherman, R. Lempicki (2008)
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resourcesNature Protocols, 4
Y. Nakano, S. Morita, A. Kawamoto, T. Suda, K. Chida, Hirotoshi Nakamura (2003)
Elevated complement C3a in plasma from patients with severe acute asthma.The Journal of allergy and clinical immunology, 112 3
P. Simonian, C. Roark, Fabian Wehrmann, Allison Lanham, F. Valle, W. Born, R. O’Brien, A. Fontenot (2009)
Th17-Polarized Immune Response in a Murine Model of Hypersensitivity Pneumonitis and Lung Fibrosis1The Journal of Immunology, 182
J. Douwes, P. Gibson, J. Pekkanen, N. Pearce (2002)
Non-eosinophilic asthma: importance and possible mechanismsThorax, 57
Leonie Rijt, H. Kuipers, N. Vos, D. Hijdra, H. Hoogsteden, B. Lambrecht (2004)
A rapid flow cytometric method for determining the cellular composition of bronchoalveolar lavage fluid cells in mouse models of asthma.Journal of immunological methods, 288 1-2
A. Kamath, I. Pavord, P. Ruparelia, E. Chilvers (2005)
Creatine supplementation as an exercise performance enhancer for patients with COPD? An idea to run withThorax, 60
T. Hashimoto, K. Akiyama, Noriaki Kobayashi, A. Mori (2005)
Comparison of IL-17 Production by Helper T Cells among Atopic and Nonatopic Asthmatics and Control SubjectsInternational Archives of Allergy and Immunology, 137
L. Mckinley, J. Alcorn, Alanna Peterson, Rachel Dupont, S. Kapadia, A. Logar, Adam Henry, C. Irvin, J. Piganelli, A. Ray, J. Kolls (2008)
TH17 Cells Mediate Steroid-Resistant Airway Inflammation and Airway Hyperresponsiveness in Mice1The Journal of Immunology, 181
N. Krug, T. Tschernig, V. Erpenbeck, J. Hohlfeld, J. Köhl (2001)
Complement factors C3a and C5a are increased in bronchoalveolar lavage fluid after segmental allergen provocation in subjects with asthma.American journal of respiratory and critical care medicine, 164 10 Pt 1
D. Bullens, Els Truyen, Liesbeth Coteur, E. Dilissen, P. Hellings, L. Dupont, J. Ceuppens (2006)
IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx?Respiratory Research, 7
Waldo Mattos, Sam Lim, R. Russell, A. Jatakanon, K. Chung, P. Barnes (2002)
Matrix metalloproteinase-9 expression in asthma: effect of asthma severity, allergen challenge, and inhaled corticosteroids.Chest, 122 5
P. Chanez, S. Wenzel, G. Anderson, J. Anto, E. Bel, L. Boulet, C. Brightling, W. Busse, M. Castro, B. Dahlén, S. Dahlén, L. Fabbri, S. Holgate, M. Humbert, M. Gaga, G. Joos, B. Levy, K. Rabe, P. Sterk, S. Wilson, I. Vachier (2007)
Severe asthma in adults: what are the important questions?The Journal of allergy and clinical immunology, 119 6
Amrita Joshi, Daniel Fong, Sameer Oak, G. Trujillo, K. Flaherty, F. Martinez, C. Hogaboam (2009)
Interleukin-17-mediated immunopathogenesis in experimental hypersensitivity pneumonitis.American journal of respiratory and critical care medicine, 179 8
J. Shannon, P. Ernst, Y. Yamauchi, R. Olivenstein, C. Lemière, S. Foley, Leo Cicora, M. Ludwig, Q. Hamid, James Martin (2008)
Differences in airway cytokine profile in severe asthma compared to moderate asthma.Chest, 133 2
S. Holgate (2008)
Pathogenesis of AsthmaClinical & Experimental Allergy, 38
M. Keir, Loise Francisco, A. Sharpe (2007)
PD-1 and its ligands in T-cell immunity.Current opinion in immunology, 19 3
C. Lloyd, Z. Brown (2006)
Chemokine ReceptorsTreatments in Respiratory Medicine, 5
J. Fahy (2009)
Eosinophilic and neutrophilic inflammation in asthma: insights from clinical studies.Proceedings of the American Thoracic Society, 6 3
K. Vermaelen, R. Pauwels (2004)
Accurate and simple discrimination of mouse pulmonary dendritic cell and macrophage populations by flow cytometry: Methodology and new insightsCytometry Part A, 61A
A. Lindén (2001)
Role of Interleukin-17 and the Neutrophil in AsthmaInternational Archives of Allergy and Immunology, 126
C. Bonnans, P. Chanez, C. Chavis (2004)
Lipoxins in asthma: potential therapeutic mediators on bronchial inflammation?Allergy, 59
B. Levy, G. Sanctis, P. Devchand, Eugene Kim, K. Ackerman, Birgitta Schmidt, W. Szczeklik, J. Drazen, C. Serhan (2002)
Multi-pronged inhibition of airway hyper-responsiveness and inflammation by lipoxin A4Nature Medicine, 8
A. Corbaz, T. Hove, S. Herren, P. Graber, B. Schwartsburd, I. Belzer, J. Harrison, T. Plitz, M. Kosco-Vilbois, Soohyun Kim, C. Dinarello, D. Novick, S. Deventer, Y. Chvatchko (2002)
IL-18-Binding Protein Expression by Endothelial Cells and Macrophages Is Up-Regulated During Active Crohn’s Disease1The Journal of Immunology, 168
Abstract Contrary to the T-helper (Th)-2 bias and eosinophil-dominated bronchial inflammation encountered in most asthmatic subjects, other patients may exhibit neutrophil-predominant asthma subphenotypes, along with Th-1 and Th-17 cells. However, the etiology of many neutrophil-dominated asthma subphenotypes remains ill-understood, in part due to a lack of appropriate experimental models. To better understand the distinct immune-pathological features of eosinophilic vs. neutrophilic asthma types, we developed an ovalbumin (OVA)-based mouse model of neutrophil-dominated allergic pulmonary inflammation. Consequently, we probed for particular inflammatory signatures and checkpoints underlying the immune pathology in this new model, as well as in a conventional, eosinophil-dominated asthma model. Briefly, mice were OVA sensitized using either aluminum hydroxide (alum) or complete Freund's adjuvants, followed by OVA aerosol challenge. T-cell, granulocyte, and inflammatory mediator profiles were determined, along with alveolar macrophage genomewide transcriptome profiling. In contrast to the Th-2-dominated phenotype provoked by alum, OVA/ complete Freund's adjuvants adjuvant-based sensitization, followed by allergen challenge, elicited a pulmonary inflammation that was poorly controlled by dexamethasone, and in which Th-1 and Th-17 cells additionally participated. Analysis of the overall pulmonary and alveolar macrophage inflammatory mediator profiles revealed remarkable similarities between both models. Nevertheless, we observed pronounced differences in the IL-12/IFN-γ axis and its control by IL-18 and IL-18 binding protein, but also in macrophage arachidonic acid metabolism and expression of T-cell instructive ligands. These differential signatures, superimposed onto a generic inflammatory signature, denote distinctive inflammatory checkpoints potentially involved in orchestrating neutrophil-dominated asthma. neutrophil-predominant asthma allergic inflammation alveolar macrophage transcriptome mouse models Footnotes Copyright © 2011 the American Physiological Society
AJP - Lung Cellular and Molecular Physiology – The American Physiological Society
Published: May 1, 2011
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