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In vitro recovery of insulin binding after downregulation in cultured normal human monocytes

In vitro recovery of insulin binding after downregulation in cultured normal human monocytes have been shown to be recycled in some studies (8, 21, 27) and degraded in others (16, 18). Although insulin receptor downregulation has been extensively studied, the biochemical changes in the receptor that cause its internalization and determine its intracellular fate are not known. Similarly, the forces driving receptor recovery are poorly understood. In this paper we describe the use of an in vitro system, cultured human monocytes, for the study of these phenomena. Human peripheral blood monocytes have been used for many studies of human insulin receptors, because of their accessibility and because they reflect changes in receptors of major insulin target tissues in disease (4). Reduction in the numbers of insulin receptors on circulating monocytes has been reported in patients with a variety of disorders, including type II diabetes (3, 24), obesity (2), and acanthosis nigricans (9). Concentrations of receptors on circulating monocytes may also correlate with glucose tolerance (5). The processes that control insulin receptors in these cells are therefore of interest and may serve as a model system to elucidate the general phenomenon of receptor control. We have shown previously that the binding capacity and affinity of insulin receptors of human monocytes are virtually http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Endocrinology and Metabolism The American Physiological Society

In vitro recovery of insulin binding after downregulation in cultured normal human monocytes

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Publisher
The American Physiological Society
Copyright
Copyright © 1985 the American Physiological Society
ISSN
0193-1849
eISSN
1522-1555
Publisher site
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Abstract

have been shown to be recycled in some studies (8, 21, 27) and degraded in others (16, 18). Although insulin receptor downregulation has been extensively studied, the biochemical changes in the receptor that cause its internalization and determine its intracellular fate are not known. Similarly, the forces driving receptor recovery are poorly understood. In this paper we describe the use of an in vitro system, cultured human monocytes, for the study of these phenomena. Human peripheral blood monocytes have been used for many studies of human insulin receptors, because of their accessibility and because they reflect changes in receptors of major insulin target tissues in disease (4). Reduction in the numbers of insulin receptors on circulating monocytes has been reported in patients with a variety of disorders, including type II diabetes (3, 24), obesity (2), and acanthosis nigricans (9). Concentrations of receptors on circulating monocytes may also correlate with glucose tolerance (5). The processes that control insulin receptors in these cells are therefore of interest and may serve as a model system to elucidate the general phenomenon of receptor control. We have shown previously that the binding capacity and affinity of insulin receptors of human monocytes are virtually

Journal

AJP - Endocrinology and Metabolism – The American Physiological Society

Published: Jul 1, 1985

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