tion of the plasma fraction with intracellular fragments (e.g., er depot vesicles) as well as with vesicles of non tissue origin such as nerves and blood vessels is likely to occur. Evidence has accumulated to support the notion that insulin and contraction stimulated in skeletal is at least partially due to a redistribution of the GLUT-4 er isoform from apparent intracellular compartments to the plasma (for review, see Ref. 17). However, it is not clear whether insulin and contractions recruit GLUT-4 ers from the same or from different intracellular storage sites (5,8). Furthermore, the mechanisms activated in the exocytotic like translocation of GLUT-4 induced by insulin and contractions is virtually unknown. We here report a new approach to obtain plasma vesicles from skeletal in sufficient amounts to allow studies of . A unique feature of the preparation in addition to the size of the vesicles is the very small contamination of the plasma with other s, allowing characteristics of pure sarcolemma to be studied. Furthermore, the effect of insulin and/or contractions in vivo on vesicle is reported. MATERIALS AND METHODS of regulation in skeletal have employed the use of isolated vesicles (5, 8-10, 16, 17, 24, 32). However,
AJP - Endocrinology and Metabolism – The American Physiological Society
Published: Feb 1, 1993
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