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Glucose metabolism by adult hepatocytes in primary culture and by cell lines from rat liver

Glucose metabolism by adult hepatocytes in primary culture and by cell lines from rat liver , D. MONTGOMERY, GERRI A. MINA J. adult hepatocytes in culture eel2 lines from rat . Am. J. Physiol. 234(3): C122-C130, 1978 or Am. J. Physiol.: Cell Physiol. 3(Z): CI22-C130, 1978. -The metabolic fate of [U-14C] has been examined in detail in adult rat hepatocytes in monolayer culture, as well as in two permanent cell linesBuffalo rat (BRL) transplantable rat hepatoma (HTC) -derived from normal rat from rat hepatoma, respectively. Under defined conditions of incubation, at a concentration of 5.5 mM, the three types of exhibited pronounced differences in . cultures, like the intact , differed from the cell lines in consuming relatively small amounts of converting approximately 50% of the total metabolized to lactate. contrast, the permanent cell lines consumed at a 40-fold greater rate than did cultures, converting 80-90% of the carbohydrate to lactate. Oxidative of carbon also differed among the three types of culture. Of the total [W4C] consumed, cultures converted approximately 30% to labeled CO, per hour, whereas the cell lines converted 5-10%. Finally, in culture exhibited adaptation as hepatucytes aged in culture, shifting progressively toward the pattern exhibited the permanent cell lines. This change occurred over a time course similar to that for http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

Glucose metabolism by adult hepatocytes in primary culture and by cell lines from rat liver

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Publisher
The American Physiological Society
Copyright
Copyright © 1978 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
Publisher site
See Article on Publisher Site

Abstract

, D. MONTGOMERY, GERRI A. MINA J. adult hepatocytes in culture eel2 lines from rat . Am. J. Physiol. 234(3): C122-C130, 1978 or Am. J. Physiol.: Cell Physiol. 3(Z): CI22-C130, 1978. -The metabolic fate of [U-14C] has been examined in detail in adult rat hepatocytes in monolayer culture, as well as in two permanent cell linesBuffalo rat (BRL) transplantable rat hepatoma (HTC) -derived from normal rat from rat hepatoma, respectively. Under defined conditions of incubation, at a concentration of 5.5 mM, the three types of exhibited pronounced differences in . cultures, like the intact , differed from the cell lines in consuming relatively small amounts of converting approximately 50% of the total metabolized to lactate. contrast, the permanent cell lines consumed at a 40-fold greater rate than did cultures, converting 80-90% of the carbohydrate to lactate. Oxidative of carbon also differed among the three types of culture. Of the total [W4C] consumed, cultures converted approximately 30% to labeled CO, per hour, whereas the cell lines converted 5-10%. Finally, in culture exhibited adaptation as hepatucytes aged in culture, shifting progressively toward the pattern exhibited the permanent cell lines. This change occurred over a time course similar to that for

Journal

AJP - Cell PhysiologyThe American Physiological Society

Published: Mar 1, 1978

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