Generation of nitric oxide and superoxide during reperfusion after focal cerebral ischemia in rats

Generation of nitric oxide and superoxide during reperfusion after focal cerebral ischemia in rats EIJI KUMURA, TOSHIKI YOSHIMINE, KO-ICHI IWATSUKI, SATONORI TANAKA, TORU HAYAKAWA, TAKESHI SHIGA, Departments of Physiology and Neurosurgery, Osaka University Kumura, Eiji, Toshiki Yoshimine, Ko-Ichi Iwatsuki, Kazunori Yamanaka, Satonori Tanaka, Toru Hayakawa, Takeshi Shiga, and Hiroaki Kosaka. Generation of nitric oxide and superoxide during reperfusion after focal in rats. Am. J. Physiol. 270 (CeZZ PhysioZ. 39): C748-C752,1996.-We investigated the levels of nitrosyl hemoglobin (HbNO) in rat jugular blood by electron spin resonance (ESR) spectroscopy during and after middle artery occlusion. The levels of plasma nitric oxide (NO) end products, nitrite plus nitrate, were compared with the levels of HbNO. Small amounts of HbNO were detected in sham-operated rats (n = 4) and those subjected to 2 h of occlusion (n = 4), whereas nitrite plus nitrate was increased only in the latter (P < 0.01; vs. sham). Upon reperfusion after 2 h of occlusion, both Hb nitrite plus nitrate clearly increased after 15 min (n = 4) and 30 min (n = 6) reperfusion (P < 0.01; vs. occlusion). Administration of superoxide dismutase (5 mg/kg) significantly increased HbNO (P < 0.05) but not plasma nitrite plus nitrate (n = 5). The increase in Hb nitrite plus nitrate after 30 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

Generation of nitric oxide and superoxide during reperfusion after focal cerebral ischemia in rats

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Publisher
The American Physiological Society
Copyright
Copyright © 1996 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
Publisher site
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Abstract

EIJI KUMURA, TOSHIKI YOSHIMINE, KO-ICHI IWATSUKI, SATONORI TANAKA, TORU HAYAKAWA, TAKESHI SHIGA, Departments of Physiology and Neurosurgery, Osaka University Kumura, Eiji, Toshiki Yoshimine, Ko-Ichi Iwatsuki, Kazunori Yamanaka, Satonori Tanaka, Toru Hayakawa, Takeshi Shiga, and Hiroaki Kosaka. Generation of nitric oxide and superoxide during reperfusion after focal in rats. Am. J. Physiol. 270 (CeZZ PhysioZ. 39): C748-C752,1996.-We investigated the levels of nitrosyl hemoglobin (HbNO) in rat jugular blood by electron spin resonance (ESR) spectroscopy during and after middle artery occlusion. The levels of plasma nitric oxide (NO) end products, nitrite plus nitrate, were compared with the levels of HbNO. Small amounts of HbNO were detected in sham-operated rats (n = 4) and those subjected to 2 h of occlusion (n = 4), whereas nitrite plus nitrate was increased only in the latter (P < 0.01; vs. sham). Upon reperfusion after 2 h of occlusion, both Hb nitrite plus nitrate clearly increased after 15 min (n = 4) and 30 min (n = 6) reperfusion (P < 0.01; vs. occlusion). Administration of superoxide dismutase (5 mg/kg) significantly increased HbNO (P < 0.05) but not plasma nitrite plus nitrate (n = 5). The increase in Hb nitrite plus nitrate after 30

Journal

AJP - Cell PhysiologyThe American Physiological Society

Published: Mar 1, 1996

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