Abstract mRNA expression of vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF-2), and hypoxia-inducible factor (HIF) subunits HIF-1α and HIF-1β in human skeletal muscle was studied during endurance exercise at different degrees of oxygen delivery. Muscle biopsies were taken before and after 45 min of one-legged knee-extension exercise performed under conditions of nonrestricted or restricted blood flow (∼15–20% lower) at the same absolute workload. Exercise increased VEGF mRNA expression by 178% and HIF-1β by 340%, but not HIF-1α and FGF-2. No significant differences between the restricted and nonrestricted groups were observed. The exercise-induced increase in VEGF mRNA was correlated to the exercise changes in HIF-1α and HIF-1β mRNA. The changes in VEGF, HIF-1α, and HIF-1β mRNAs were correlated to the exercise-induced increase in femoral venous plasma lactate concentration. It is concluded that 1 ) VEGF but not FGF-2 gene expression is upregulated in human skeletal muscle by a single bout of dynamic exercise and that there is a graded response in VEGF mRNA expression related to the metabolic stress and 2 ) the increase in VEGF mRNA expression correlates to the changes in both HIF-1α and HIF-1β mRNA. ischemia gene expression vascular endothelial growth factor fibroblast growth factor hypoxia-inducible factor 1 Footnotes Address reprint requests to T. Gustafsson. This work was supported by the Swedish Heart-Lung Association, the Fraenckel Foundation for Medical Research, the Swedish Medical Research Council (4494), the Swedish Society of Medicine, the Swedish National Center for Research in Sports, and the Swedish Association for the Promotion of Sport. The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “ advertisement ” in accordance with 18 U.S.C. §1734 solely to indicate this fact. Copyright © 1999 the American Physiological Society
AJP - Heart and Circulatory Physiology – The American Physiological Society
Published: Feb 1, 1999
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