Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Estradiol attenuates the antidiuretic action of vasopressin in ovariectomized rats

Estradiol attenuates the antidiuretic action of vasopressin in ovariectomized rats METHODS All experimental procedures were approved by the Institutional Animal Care and Use Committee and were conducted in accordance with National Institutes of Health Guidelines for the care and use of animals. Animak. Female Sprague-Dawley rats (Harlan, Indianapolis, IN) were used. When the rats were 3 wk old, they were either sham operated or ovariectomized by the supplier. In the 6th wk, the rats were implanted subcutaneously with one of the following slow-release pellets (Innovative Research of America, Toledo, OH): vehicle (cholesterol, 0.25-25 mg), 17@estradiol (0.25 mg), progesterone (25 mg), or 17pestradiol (0.25 mg) plus progesterone (25 mg). Because these pellets provide a steady release of the hormone for a 21-day period, the old pellets were removed and replaced by corresponding new ones in the 9th wk. The doses of the new pellets were doubled to take into consideration the weight gain of the rats. These doses have been reported to achieve plasma concentrations of estradiol and progesterone two to four times those seen in rats in the proestrus phase of the estrus cycle (1, 26). The rats were housed individually with free access to food and tap water in a room with controlled temperature (24°C) and lighting http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Regulatory, Integrative and Comparative Physiology The American Physiological Society

Estradiol attenuates the antidiuretic action of vasopressin in ovariectomized rats

Download PDF
7 pages

Loading next page...
 
/lp/the-american-physiological-society/estradiol-attenuates-the-antidiuretic-action-of-vasopressin-in-mKv00JJqJf

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
The American Physiological Society
Copyright
Copyright © 1995 the American Physiological Society
ISSN
0363-6119
eISSN
1522-1490
Publisher site
See Article on Publisher Site

Abstract

METHODS All experimental procedures were approved by the Institutional Animal Care and Use Committee and were conducted in accordance with National Institutes of Health Guidelines for the care and use of animals. Animak. Female Sprague-Dawley rats (Harlan, Indianapolis, IN) were used. When the rats were 3 wk old, they were either sham operated or ovariectomized by the supplier. In the 6th wk, the rats were implanted subcutaneously with one of the following slow-release pellets (Innovative Research of America, Toledo, OH): vehicle (cholesterol, 0.25-25 mg), 17@estradiol (0.25 mg), progesterone (25 mg), or 17pestradiol (0.25 mg) plus progesterone (25 mg). Because these pellets provide a steady release of the hormone for a 21-day period, the old pellets were removed and replaced by corresponding new ones in the 9th wk. The doses of the new pellets were doubled to take into consideration the weight gain of the rats. These doses have been reported to achieve plasma concentrations of estradiol and progesterone two to four times those seen in rats in the proestrus phase of the estrus cycle (1, 26). The rats were housed individually with free access to food and tap water in a room with controlled temperature (24°C) and lighting

Journal

AJP - Regulatory, Integrative and Comparative Physiology – The American Physiological Society

Published: Apr 1, 1995

There are no references for this article.