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Effects of EDCF and endothelin on phosphatidylinositol hydrolysis and contraction in rat aorta

Effects of EDCF and endothelin on phosphatidylinositol hydrolysis and contraction in rat aorta All three isoforms of endothelin elicit contractions of arterial vessels, in vitro, that are strikingly similar to those observed by Hickey et al. (16), using EDCF present in conditioned media from cultured bovine aortic endothelial cells. Both EDCF from cultured cells and endothelin induce slowly developing tonic contractions that are dependent on extracellular Ca’+, are reduced by dihydropyridine Ca2+ channel antagonists, but are not affected by antagonists of CYand ,8- adrenergic receptors, histamine HI-receptors, angiotensin II receptors, serotonergic receptors, cyclooxygenase, or lipooxygenase (13, 16, 17, 19, 28, 41). These data suggest that the vasoconstriction induced by endothelin and EDCF is a response to the same molecule. However, to what extent endothelin accounts for all of the vasoconstricting activity of endothelial cell conditioned medium remains uncertain. For this reason, we have evaluated and compared the mechanisms of action of three different prepaions, purified synthetic endothelin (ET- 1)) whole endothelial cell conditioned medium containing all s vasoactive substances released by cultured endothelial cells (EDCF), and a partially purified prepaion of EDCF. The present study focuses on the effects of these prepaions on phosphatidylinositide hydrolysis in the intact . Some of the results have been presented in preliminary form (18-20). MATERIALS http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

Effects of EDCF and endothelin on phosphatidylinositol hydrolysis and contraction in rat aorta

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Publisher
The American Physiological Society
Copyright
Copyright © 1990 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
Publisher site
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Abstract

All three isoforms of endothelin elicit contractions of arterial vessels, in vitro, that are strikingly similar to those observed by Hickey et al. (16), using EDCF present in conditioned media from cultured bovine aortic endothelial cells. Both EDCF from cultured cells and endothelin induce slowly developing tonic contractions that are dependent on extracellular Ca’+, are reduced by dihydropyridine Ca2+ channel antagonists, but are not affected by antagonists of CYand ,8- adrenergic receptors, histamine HI-receptors, angiotensin II receptors, serotonergic receptors, cyclooxygenase, or lipooxygenase (13, 16, 17, 19, 28, 41). These data suggest that the vasoconstriction induced by endothelin and EDCF is a response to the same molecule. However, to what extent endothelin accounts for all of the vasoconstricting activity of endothelial cell conditioned medium remains uncertain. For this reason, we have evaluated and compared the mechanisms of action of three different prepaions, purified synthetic endothelin (ET- 1)) whole endothelial cell conditioned medium containing all s vasoactive substances released by cultured endothelial cells (EDCF), and a partially purified prepaion of EDCF. The present study focuses on the effects of these prepaions on phosphatidylinositide hydrolysis in the intact . Some of the results have been presented in preliminary form (18-20). MATERIALS

Journal

AJP - Cell Physiology – The American Physiological Society

Published: Jan 1, 1990

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