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Effect of histamine on the basolateral K+ conductance of frog stomach oxyntic cells and surface epithelial cells

Effect of histamine on the basolateral K+ conductance of frog stomach oxyntic cells and surface epithelial cells METHODS THE IMPORTANCE K+ in ion transport is now well established, both in amphibian stomach in vitro (6, 14) and in isolated rabbit glands (1). Initially, Rehm and co-workers (12, 14) demonstrated that removal external K+ blocked H+ secretion and addition K+ to one the bathing solutions was able to restore it. They concluded that a critical level free K+ in the acid-secreting was essential for H+ secretion (see also Ref. 18). Today, we know that K+ is required for operation the H+-K+ pump, which is located in the apical membrane the oxyntic and secretes H+ in exchange for K+ (9, 13). Since K ions are not freely available in the gland lumen, they recirculate from the cell via a passive pathway. This K+ has been identified in membrane vesicles parietal , which had been isolated from after stimulation by histamine (2, 20). Despite this evidence for the importance K+ in secretion little is known about K+ transport 0193~1857/90 $1.50 Copyright The experiments were performed on fundus Rana esculenta. The s were kept unfed for up to 10 days in an aquarium at room temperature. After the animals were killed and the stomachs removed, the fundus was separated http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Gastrointestinal and Liver Physiology The American Physiological Society

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