Abstract Mechanisms in which p -coumaric acid (CA) acts as an antioxidant are not well understood. This study investigated whether CA can act as a direct scavenger of reactive oxygen species (ROS) and whether it minimizes the oxidation of low-density lipoprotein (LDL). Rats were administered CA in drinking water at low or high doses for 10, 21, and 30 days (uptakes were 29 and 317 mg/day, respectively). Blood levels of 8-epiprostaglandin F 2α were monitored as a marker of LDL oxidation. Oral administration of CA (317 mg/day) for 30 days significantly inhibited LDL oxidation. CA also reduced LDL cholesterol levels in serum but had no effect on levels of high-density lipoprotein cholesterol. In vitro studies that used electron spin resonance in combination with spin trapping techniques were used to determine the ability of CA to scavenge ROS and alter LDL oxidation. CA effectively scavenged ·OH in a dose-dependent manner. IC 50 and maximum velocity for CA scavenging of ·OH were 4.72 μM and 1.2 μM/s, respectively, with a rate constant of 1.8 × 10 11 M −1 · s −1 . Our studies suggest that the antioxidant properties of CA may involve the direct scavenging of ROS such as ·OH. hydroxyl radical lipid peroxidation reactive oxygen species Footnotes Address for reprint requests and other correspondence: V. Vallyathan, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd., Morgantown, WV 26505-2888 (E-mail: firstname.lastname@example.org ). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “ advertisement ” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Copyright © 2000 the American Physiological Society
AJP - Cell Physiology – The American Physiological Society
Published: Oct 1, 2000
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