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Direct measurement of intracellular pressure

Direct measurement of intracellular pressure S. M. KELLY AND P. T. MACKLEM Meakins-Christie Laboratories, McGill University Clinic and Royal Victoria Hospital, Montreal, Quebec HZX 2P2, Canada KELLY, S. M., AND P. T. MACKLEM. Direct . Am. J. Physiol. 260 (Cell Physiol. 29): C652-C657,1991.-The feasibility using the servo-null technique for direct (Pin) was investigated. A large cell, the Xenopus laevis oocyte, was chosen for study, and it was established that Pi, obtained with this method was both stable and accurate in these cells. Median resting Pi, in oocytes was found to be 0.27 cmH20, range 0.140.61 cmH20. During osmotic swelling Pi, increased, in a nonlinear fashion, to a value 4.11 cmH20, range 2.61-8.91 cmHzO, with increases in cell volume (V,) 24 t 3% (SE). This technique may be use in the study cellular mechanics. cell mechanics; oocytes micro; osmotic swelling; Xenopus laevis METHODS (Pi,)can be estimatedindirectly by a number techniques. These include aspiration part the cell into a pipette (18, 19, 23, 25-27), the sessile drop technique (16, 17), compression the cell (3, 18, 31), and stretching (24) or centrifugation (15) cells. These methods are time consuming, since Pi, is usually determined by extrapolation back to the nonstressed situation and, as a result, it http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

Direct measurement of intracellular pressure

S. M. Kelly and P. T. Macklem
AJP - Cell Physiology , Volume 260: C652 – Mar 1, 1991
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Publisher
The American Physiological Society
Copyright
Copyright © 1991 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
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Abstract

S. M. KELLY AND P. T. MACKLEM Meakins-Christie Laboratories, McGill University Clinic and Royal Victoria Hospital, Montreal, Quebec HZX 2P2, Canada KELLY, S. M., AND P. T. MACKLEM. Direct . Am. J. Physiol. 260 (Cell Physiol. 29): C652-C657,1991.-The feasibility using the servo-null technique for direct (Pin) was investigated. A large cell, the Xenopus laevis oocyte, was chosen for study, and it was established that Pi, obtained with this method was both stable and accurate in these cells. Median resting Pi, in oocytes was found to be 0.27 cmH20, range 0.140.61 cmH20. During osmotic swelling Pi, increased, in a nonlinear fashion, to a value 4.11 cmH20, range 2.61-8.91 cmHzO, with increases in cell volume (V,) 24 t 3% (SE). This technique may be use in the study cellular mechanics. cell mechanics; oocytes micro; osmotic swelling; Xenopus laevis METHODS (Pi,)can be estimatedindirectly by a number techniques. These include aspiration part the cell into a pipette (18, 19, 23, 25-27), the sessile drop technique (16, 17), compression the cell (3, 18, 31), and stretching (24) or centrifugation (15) cells. These methods are time consuming, since Pi, is usually determined by extrapolation back to the nonstressed situation and, as a result, it

Journal

AJP - Cell Physiology – The American Physiological Society

Published: Mar 1, 1991

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