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Differences between cytoplasmic surfaces of deep-etched heart and liver gap junctions

Differences between cytoplasmic surfaces of deep-etched heart and liver gap junctions C. K. MANJUNATH, AND ERNEST PAGE of Anatomy, of Medicine, Kyushu University, Fukuoka 812, Japan; and University of Chicago, Chicago, Illinois 60637 SHIBATA, YOSABURO, C. K. MANJUNATH, AND ERNEST PAGE. Differences between cytoplasmic surfaces of deep-etched heart artd liver gap junctions. Am. J. Physiol. 249 (Heart Circ. Physiol. 18): H690-H693,1985.---We have comparedthe ultrastructures of the cytoplasmic surfaces(CS) of isolated, glutaraldehyde-fixed gap junctional pellets from rat ventricles and liver by rapid freezing on a liquid helium-cooledsurface, freeze fracture, deep etching, and double-axis rotary replication (J. Microsc. Oxford 137: 121-123, 1984). Deep-etched unproteolyzed cardiac junctions [protein subunit relative molecular wt (A&) 44,000-47,OOO], isolated with phenylmethylsulfonylfluoride (PMSF) [Am. J. Physiol. 246 (Heart Circ. Physiol. 15): H865-H875,1984; C. K. Manjunath, G. E. Goings, and E. Page. Proteolysis of cardiac gap junctions during their isolation from rat hearts. J. Membr. Bill. In press.] had particulate CS, while proteolyzed cardiac junctions (subunit M, 29,500)madewithout PMSF and liver junctions (M, 28,000) made with or without PMSF had nonparticulate CS. Taken together with our previous findings that electron micrographsof thin-sectioned isolated unproteolyzed cardiac junctions have urea-resistant fuzzy CS coatings [Am. J. Physiol. 246 (Heart Circ. Physiol. 15): H865-H875j 19841,that proteolyzed cardiac junctions and isolated liver junctions http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Heart and Circulatory Physiology The American Physiological Society

Differences between cytoplasmic surfaces of deep-etched heart and liver gap junctions

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Publisher
The American Physiological Society
Copyright
Copyright © 1985 the American Physiological Society
ISSN
0363-6135
eISSN
1522-1539
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Abstract

C. K. MANJUNATH, AND ERNEST PAGE of Anatomy, of Medicine, Kyushu University, Fukuoka 812, Japan; and University of Chicago, Chicago, Illinois 60637 SHIBATA, YOSABURO, C. K. MANJUNATH, AND ERNEST PAGE. Differences between cytoplasmic surfaces of deep-etched heart artd liver gap junctions. Am. J. Physiol. 249 (Heart Circ. Physiol. 18): H690-H693,1985.---We have comparedthe ultrastructures of the cytoplasmic surfaces(CS) of isolated, glutaraldehyde-fixed gap junctional pellets from rat ventricles and liver by rapid freezing on a liquid helium-cooledsurface, freeze fracture, deep etching, and double-axis rotary replication (J. Microsc. Oxford 137: 121-123, 1984). Deep-etched unproteolyzed cardiac junctions [protein subunit relative molecular wt (A&) 44,000-47,OOO], isolated with phenylmethylsulfonylfluoride (PMSF) [Am. J. Physiol. 246 (Heart Circ. Physiol. 15): H865-H875,1984; C. K. Manjunath, G. E. Goings, and E. Page. Proteolysis of cardiac gap junctions during their isolation from rat hearts. J. Membr. Bill. In press.] had particulate CS, while proteolyzed cardiac junctions (subunit M, 29,500)madewithout PMSF and liver junctions (M, 28,000) made with or without PMSF had nonparticulate CS. Taken together with our previous findings that electron micrographsof thin-sectioned isolated unproteolyzed cardiac junctions have urea-resistant fuzzy CS coatings [Am. J. Physiol. 246 (Heart Circ. Physiol. 15): H865-H875j 19841,that proteolyzed cardiac junctions and isolated liver junctions

Journal

AJP - Heart and Circulatory Physiology – The American Physiological Society

Published: Sep 1, 1985

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