Depot differences in steroid receptor expression in adipose tissue: possible role of the local steroid milieu

Depot differences in steroid receptor expression in adipose tissue: possible role of the local... Sex hormones play an important role in adipose tissue metabolism by activating specific receptors that alter several steps of the lipolytic and lipogenic signal cascade in depot- and sex-dependent manners. However, studies focusing on steroid receptor status in adipose tissue are scarce. In the present study, we analyzed steroid content testosterone (T), 17 -estradiol (17 -E 2 ), and progesterone (P 4 ) and steroid receptor mRNA levels in different rat adipose tissue depots. As expected, T levels were higher in males than in females ( P = 0.031), whereas the reverse trend was observed for P 4 ( P < 0.001). It is noteworthy that 17 -E 2 adipose tissue levels were higher in inguinal than in the rest of adipose tissues for both sexes, where no sex differences in 17 -E 2 tissue levels were noted ( P = 0.010 for retroperitoneal, P = 0.005 for gonadal, P = 0.018 for mesenteric). Regarding steroid receptor levels, androgen (AR) and estrogen receptor (ER)α and ER densities were more clearly dependent on adipose depot location than on sex, with visceral depots showing overall higher mRNA densities than their subcutaneous counterparts. Besides, expression of ERα predominated over ER expression, and progesterone receptor (PR-B form and PR-A+B form) mRNAs were identically expressed regardless of anatomic depot and sex. In vitro studies in 3T3-L1 cells showed that 17 -E 2 increased ERα ( P = 0.001) and AR expression ( P = 0.001), indicating that estrogen can alter estrogenic and androgenic signaling in adipose tissue. The results highlighted in this study demonstrate important depot-dependent differences in the sensitivity of adipose tissues to sex hormones between visceral and subcutaneous depots that could be related to metabolic situations observed in response to sex hormones. steroid receptors; testosterone; 17 -estradiol; progesterone Address for reprint requests and other correspondence: P. Roca, Departament de Biologia Fonamental i Ciències de la Salut, Ed. Guillem Colom, Universitat de les Illes Balears, Cra Valldemossa, Km 7.5, CP 07122, Palma de Mallorca, Balearic Islands, Spain (E-mail: pilar.roca@uib.es ) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Endocrinology and Metabolism The American Physiological Society

Depot differences in steroid receptor expression in adipose tissue: possible role of the local steroid milieu

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Publisher
The American Physiological Society
Copyright
Copyright © 2011 the American Physiological Society
ISSN
0193-1849
eISSN
1522-1555
DOI
10.1152/ajpendo.00270.2004
Publisher site
See Article on Publisher Site

Abstract

Sex hormones play an important role in adipose tissue metabolism by activating specific receptors that alter several steps of the lipolytic and lipogenic signal cascade in depot- and sex-dependent manners. However, studies focusing on steroid receptor status in adipose tissue are scarce. In the present study, we analyzed steroid content testosterone (T), 17 -estradiol (17 -E 2 ), and progesterone (P 4 ) and steroid receptor mRNA levels in different rat adipose tissue depots. As expected, T levels were higher in males than in females ( P = 0.031), whereas the reverse trend was observed for P 4 ( P < 0.001). It is noteworthy that 17 -E 2 adipose tissue levels were higher in inguinal than in the rest of adipose tissues for both sexes, where no sex differences in 17 -E 2 tissue levels were noted ( P = 0.010 for retroperitoneal, P = 0.005 for gonadal, P = 0.018 for mesenteric). Regarding steroid receptor levels, androgen (AR) and estrogen receptor (ER)α and ER densities were more clearly dependent on adipose depot location than on sex, with visceral depots showing overall higher mRNA densities than their subcutaneous counterparts. Besides, expression of ERα predominated over ER expression, and progesterone receptor (PR-B form and PR-A+B form) mRNAs were identically expressed regardless of anatomic depot and sex. In vitro studies in 3T3-L1 cells showed that 17 -E 2 increased ERα ( P = 0.001) and AR expression ( P = 0.001), indicating that estrogen can alter estrogenic and androgenic signaling in adipose tissue. The results highlighted in this study demonstrate important depot-dependent differences in the sensitivity of adipose tissues to sex hormones between visceral and subcutaneous depots that could be related to metabolic situations observed in response to sex hormones. steroid receptors; testosterone; 17 -estradiol; progesterone Address for reprint requests and other correspondence: P. Roca, Departament de Biologia Fonamental i Ciències de la Salut, Ed. Guillem Colom, Universitat de les Illes Balears, Cra Valldemossa, Km 7.5, CP 07122, Palma de Mallorca, Balearic Islands, Spain (E-mail: pilar.roca@uib.es )

Journal

AJP - Endocrinology and MetabolismThe American Physiological Society

Published: Jan 1, 2005

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