Changes in plasma protein extravasation in rat skin during inflammatory challenges evaluated by microdialysis

Changes in plasma protein extravasation in rat skin during inflammatory challenges evaluated by... Docetaxel and prostaglandin E 1 (PGE 1 ) increase transcapillary albumin extravasation and reduce interstitial fluid pressure in the skin. In this study the microdialysate concentration (C m ) of 125 I-labeled human serum albumin ( 125 I-HSA) and different-sized endogenous plasma proteins (EPP) was compared to evaluate changes in transcapillary extravasation of plasma proteins. 125 I-HSA was also used to estimate changes in the specific activity of albumin. Extravasation of 125 I-HSA and EPP from plasma to interstitium in the rat skin was compared during continuous administration of docetaxel and PGE 1 by using microdialysis in anesthetized rats. Also, 20 ml of Ringer solution (RS) were injected intravenously during 10 min in a separate group. Two hollow plasmapheresis fibers (3 cm, cut off 3,000 kDa), one acting as control, were placed subcutaneously on the back skin and perfused with RS (5 µl/min, 140 min, collected every 10 min). The size of the different EPP was estimated to be 73, 65, 56, 47, and 39 Å, separated by a size-exclusion high-performance liquid chromatography column and quantified by UV detection (280 nm). Docetaxel (0.5 mg/ml, n = 5) increased C m of 125 I-HSA and EPP of sizes 73, 65, 56, and 39 Å significantly ( P < 0.05) compared with control. PGE 1 (20 µg/ml, n = 6) increased C m of 125 I-HSA significantly ( P < 0.05) but none of the different-sized EPP was increased compared with control. Intravenous RS (20 ml, n = 6) increased C m of 125 I-HSA and increased all the different-sized EPP significantly ( P < 0.05) compared with control. Although the microdialysis method is able to monitor qualitative changes in capillary permeability, a quantitative determination of the capillary reflection coefficient or permeability-surface area product was not possible, because steady state between plasma and dialysate was not achieved during the measurement period. The different pattern of extravasation of EPP and 125 I-HSA after docetaxel, PGE 1 , and RS indicates increased interstitial transport rate and/or increased capillary permeability after docetaxel and RS, whereas PGE 1 seems to increase transcapillary fluid flux without altering the permeability. high-performance liquid chromatography; prostaglandin E 1 ; docetaxel; transcapillary transport Address for reprint requests and other correspondence: B. Å. Borge, Dept. of Biomedicine, Section of Physiology, Univ. of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway (email: bengt.borge@biomed.uib.no ) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Heart and Circulatory Physiology The American Physiological Society

Changes in plasma protein extravasation in rat skin during inflammatory challenges evaluated by microdialysis

Loading next page...
 
/lp/the-american-physiological-society/changes-in-plasma-protein-extravasation-in-rat-skin-during-KAX8iEf2GL
Publisher
The American Physiological Society
Copyright
Copyright © 2011 the American Physiological Society
ISSN
0363-6135
eISSN
1522-1539
DOI
10.1152/ajpheart.00395.2005
Publisher site
See Article on Publisher Site

Abstract

Docetaxel and prostaglandin E 1 (PGE 1 ) increase transcapillary albumin extravasation and reduce interstitial fluid pressure in the skin. In this study the microdialysate concentration (C m ) of 125 I-labeled human serum albumin ( 125 I-HSA) and different-sized endogenous plasma proteins (EPP) was compared to evaluate changes in transcapillary extravasation of plasma proteins. 125 I-HSA was also used to estimate changes in the specific activity of albumin. Extravasation of 125 I-HSA and EPP from plasma to interstitium in the rat skin was compared during continuous administration of docetaxel and PGE 1 by using microdialysis in anesthetized rats. Also, 20 ml of Ringer solution (RS) were injected intravenously during 10 min in a separate group. Two hollow plasmapheresis fibers (3 cm, cut off 3,000 kDa), one acting as control, were placed subcutaneously on the back skin and perfused with RS (5 µl/min, 140 min, collected every 10 min). The size of the different EPP was estimated to be 73, 65, 56, 47, and 39 Å, separated by a size-exclusion high-performance liquid chromatography column and quantified by UV detection (280 nm). Docetaxel (0.5 mg/ml, n = 5) increased C m of 125 I-HSA and EPP of sizes 73, 65, 56, and 39 Å significantly ( P < 0.05) compared with control. PGE 1 (20 µg/ml, n = 6) increased C m of 125 I-HSA significantly ( P < 0.05) but none of the different-sized EPP was increased compared with control. Intravenous RS (20 ml, n = 6) increased C m of 125 I-HSA and increased all the different-sized EPP significantly ( P < 0.05) compared with control. Although the microdialysis method is able to monitor qualitative changes in capillary permeability, a quantitative determination of the capillary reflection coefficient or permeability-surface area product was not possible, because steady state between plasma and dialysate was not achieved during the measurement period. The different pattern of extravasation of EPP and 125 I-HSA after docetaxel, PGE 1 , and RS indicates increased interstitial transport rate and/or increased capillary permeability after docetaxel and RS, whereas PGE 1 seems to increase transcapillary fluid flux without altering the permeability. high-performance liquid chromatography; prostaglandin E 1 ; docetaxel; transcapillary transport Address for reprint requests and other correspondence: B. Å. Borge, Dept. of Biomedicine, Section of Physiology, Univ. of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway (email: bengt.borge@biomed.uib.no )

Journal

AJP - Heart and Circulatory PhysiologyThe American Physiological Society

Published: May 1, 2006

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off