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Calcium pump epitopes in placental trophoblast basal plasma membranes

Calcium pump epitopes in placental trophoblast basal plasma membranes BORKE,JAMES L., ARIEL CARIDE, ANIL K. VERMA,LUCKY K. KELLEY$ARL H. SMITH,JOHN T. PENNISTON,ANDRAJIV KUMAR. Calcium epitopes in trophoblast basal plasma s. Am. J. Physiol. 257 (Cell Physiol. 26): C341-C346, 1989.-The syncytiotrophoblast represents the primary cellular barrier between maternal and fetal circulations in the placenta. Large amounts of Ca” are transported across this barrier by mechanisms that are not clearly understood. To further understand this phenomenon, we examined rat and human placenta by immunohistochemical and protein blotting techniques with a monoclonal antibody raised against the human erythrocyte plasma Ca2+ . Immunohistochemistry with this antibody showed specific staining in the human placenta of the basal (fetal facing) surface of the syncytiotrophoblast. In the rat placenta, immunohistochemistry also showed specific staining of the innermost (fetal facing) layer of the trophoblast and the basal surface of the endoderm of the intra yolk sac. In Western blots of homogenates and s, the monoclonal antibody bound to a 140,000-mol wt band, characteristic of Ca2+ s in other tissues. Western blots of isolated basal s showed more intense staining than isolated microvillous s, confirming the results of the immunohistochemistry. In addition, Ca2+ transport in basal vesicles from human placenta was inhibited by polyclonal antibodies prepared http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Cell Physiology The American Physiological Society

Calcium pump epitopes in placental trophoblast basal plasma membranes

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Publisher
The American Physiological Society
Copyright
Copyright © 1989 the American Physiological Society
ISSN
0363-6143
eISSN
1522-1563
Publisher site
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Abstract

BORKE,JAMES L., ARIEL CARIDE, ANIL K. VERMA,LUCKY K. KELLEY$ARL H. SMITH,JOHN T. PENNISTON,ANDRAJIV KUMAR. Calcium epitopes in trophoblast basal plasma s. Am. J. Physiol. 257 (Cell Physiol. 26): C341-C346, 1989.-The syncytiotrophoblast represents the primary cellular barrier between maternal and fetal circulations in the placenta. Large amounts of Ca” are transported across this barrier by mechanisms that are not clearly understood. To further understand this phenomenon, we examined rat and human placenta by immunohistochemical and protein blotting techniques with a monoclonal antibody raised against the human erythrocyte plasma Ca2+ . Immunohistochemistry with this antibody showed specific staining in the human placenta of the basal (fetal facing) surface of the syncytiotrophoblast. In the rat placenta, immunohistochemistry also showed specific staining of the innermost (fetal facing) layer of the trophoblast and the basal surface of the endoderm of the intra yolk sac. In Western blots of homogenates and s, the monoclonal antibody bound to a 140,000-mol wt band, characteristic of Ca2+ s in other tissues. Western blots of isolated basal s showed more intense staining than isolated microvillous s, confirming the results of the immunohistochemistry. In addition, Ca2+ transport in basal vesicles from human placenta was inhibited by polyclonal antibodies prepared

Journal

AJP - Cell Physiology – The American Physiological Society

Published: Aug 1, 1989

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