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AKIHIRO TOJO, C. CRAIG TISHER, AND KIRSTEN M. MADSEN Division of Nephrology, Hypertension, and Transplantation, College of Medicine, University of Florida, Gainesville, Florida 32610 Tojo, Akihiro, C. Craig Tisher, and Kirsten M. Madsen. Angiotensin II regulates cortical collecting duct. Am. J. Physiol. 267 (Renal Fhid EZectroZyte PhysioZ. 36): F1045-F1051, 1994.-Angiotensin II (ANG II) plays an important role in the regulation of solute in the kidney, and its on proxim .a1 tubule transport sodium and fluid transport has been studied extensively. Although there is evidence that ANG II receptors are present also in the distal nephron and collecting duct, little is known about the physiological role II in these segments of the renal tubule. Preliminary studies in our laboratory suggest that ANG II may have both structural and functional s on intercalated cells in the cortical collecting duct (). Therefore, the present study examines the H+-adenosinetriphosphatase () and H+-K+-ATPase in individual segments microdissected from collagenase-treated rat kidneys. The was measured as bafilomycin-sensitive ATPase , and H+ -K+ATPase was measured as Sch-28080-sensitive ATPase , by a fluorometric microassay. Preincubation of segments with ANG II, 10-10-10-5 M, caused a dose-dependent decrease in with maximum inhibition at lo-â M II. Th e
AJP - Renal Physiology – The American Physiological Society
Published: Dec 1, 1994
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