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An Arg-Gly-Asp peptide stimulates Ca2+ efflux from osteoclast precursors through a novel mechanism

An Arg-Gly-Asp peptide stimulates Ca2+ efflux from osteoclast precursors through a novel mechanism KENSUKE YAMAKAWA, RANDALL DUNCAN, Renal Division, Jewish Hospital at Washington St. Louis, Missouri 63110 Yamakawa, Kensuke, Randall Duncan, and Keith A. Hruska. An Arg-Gly-Asp peptide stimulates from osteoclast precursors through a novel mechanism. Am. J. Physiol. 266 (Renal Fluid Electrolyte PhysioZ. 35): F651F657, 1994.-We examined the effect of a peptide containing the Arg-Gly-Asp () on 45 from osteoclast precursors. 45-loaded osteoclast precursors were treated with (170 PM) for 10 min after 30 min of basal perfusion with a bicarbonate-containing buffer. significantly increased fractional of from treated cells compared with vehicle-treated cells (P < 0.01) or cells treated with up to 200 pg/ml of a control peptide containing GRGESP. The effect of was sustained for 15 min after the peptide was removed from the perfusate, but control levels of returned by 1 h. The effect of was most likely due to activation of the plasma membrane -adenosinetriphosphatase (-ATPase) pump, as indicated by its inhibition with vanadate and a calmodulin antagonist, N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide, and the absence of an effect of Na+/ exchange inhibition. An inhibitor of cyclic. nucleotide-dependent protein kinases, N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (0.1 mM), failed to inhibit - . However, genistein and herbimycin A, inhibitors of protein-tyrosine kinases, blocked by . The http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Renal Physiology The American Physiological Society

An Arg-Gly-Asp peptide stimulates Ca2+ efflux from osteoclast precursors through a novel mechanism

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Publisher
The American Physiological Society
Copyright
Copyright © 1994 the American Physiological Society
ISSN
0363-6127
eISSN
1522-1466
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Abstract

KENSUKE YAMAKAWA, RANDALL DUNCAN, Renal Division, Jewish Hospital at Washington St. Louis, Missouri 63110 Yamakawa, Kensuke, Randall Duncan, and Keith A. Hruska. An Arg-Gly-Asp peptide stimulates from osteoclast precursors through a novel mechanism. Am. J. Physiol. 266 (Renal Fluid Electrolyte PhysioZ. 35): F651F657, 1994.-We examined the effect of a peptide containing the Arg-Gly-Asp () on 45 from osteoclast precursors. 45-loaded osteoclast precursors were treated with (170 PM) for 10 min after 30 min of basal perfusion with a bicarbonate-containing buffer. significantly increased fractional of from treated cells compared with vehicle-treated cells (P < 0.01) or cells treated with up to 200 pg/ml of a control peptide containing GRGESP. The effect of was sustained for 15 min after the peptide was removed from the perfusate, but control levels of returned by 1 h. The effect of was most likely due to activation of the plasma membrane -adenosinetriphosphatase (-ATPase) pump, as indicated by its inhibition with vanadate and a calmodulin antagonist, N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide, and the absence of an effect of Na+/ exchange inhibition. An inhibitor of cyclic. nucleotide-dependent protein kinases, N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (0.1 mM), failed to inhibit - . However, genistein and herbimycin A, inhibitors of protein-tyrosine kinases, blocked by . The

Journal

AJP - Renal Physiology – The American Physiological Society

Published: Apr 1, 1994

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