FOLLMANN, WOLFRAM, ERNST PETZINGER, AND ROLF K. H. KINNE. Alterations of bile acid and bumetanide uptake during culturing of rat hepatocytes. Am. J. Physiol. 258 (Cell Physiol. 27): C700-C712, 1990.-Uptake by the multispecific bile acid transport system of [3H]taurocholate, [14C]cholate, and [âHIbumetanide into primary cultures of rat hepatocytes was compared with their uptake into freshly isolated rat hepatocytes. The uptake maximum vecity ( V,,,) of all compounds declined in primary culture, whereas the Michaelis constant (&) values remained stable. ss of uptake was not due to the reduction of driving forces as evaluated from the level of ATP and the activity of Naâ-Kâ-ATPase. No a-fetoprotein was detectable in culture supernatants. Neither growth factors (glycylhistidyllysine, epidermal growth factor), peroxisome and cell proliferators (nafenopin, dimethyl sulfoxide), nor bile acids prevented the ss of transport in hepatocyte culture. However, addition of dibutyryl adenosine 3â5â-cyclic monophosphate protracted the transport activity significantly. When cultured rat hepatocytes with reduced transport were detached by trypsin, cells rounded up and showed the same uptake capacity for bumetanide, cholate, and taurocholate as seen in freshly isolated hepatocytes. âCrypticâ transport activity in the wer basolateral membrane facing the support was found using an incubation chamber for cultured hepatocytes,
AJP - Cell Physiology – The American Physiological Society
Published: Apr 1, 1990
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera