METHODS Animal Selection and Experimental Design Eighty-seven male Sprague-Dawley rats (245300 g, Simonson Laboraries) were housed in pairs in animal quarters where light (light, 0700-1900 h) and temperature (22-24Â°C) were aumatically controlled. The animals were randomly assigned one of two experimental groups. The LCD group consumed a high in fat and low in carbohydrate (78% fat, 21% protein, 1% carbohydrate by caloric value). The normal- group (ND) consumed a normal (11% fat, 20% protein, 69% carbohydrate by caloric value). Both s were supplemented with vitamins and minerals (see 1). After either 1 or 5 wk on the s, rats from each group were killed either before (resting controls) or after an exhausting run on a rodent treadmill at 35 m.min-â and 0% grade. The experimental groups were identified according (LCD or ND), duration of (1 or 5 wk), and exercise condition [rested (R) or exhausted (EX)]. During the experiment, rats had access food and water ad libitum. acclimate the animals running on the treadmill, all animals, regardless of group, were exercised at 35 momin-l and 0% grade for 10 min a day, 5 days/ wk for 3 wk before being killed. Exercise and Killing Procedures Because of possible
Journal of Applied Physiology – The American Physiological Society
Published: Jan 1, 1984
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