Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Bishop, S. Blair, A. Chagovetz (2006)
A competitive kinetic model of nucleic acid surface hybridization in the presence of point mutants.Biophysical journal, 90 3
K. Pappaert, G. Desmet (2006)
A dimensionless number analysis of the hybridization process in diffusion- and convection-driven DNA microarray systems.Journal of biotechnology, 123 4
K. Jensen, H. Ørum, P. Nielsen, B. Nordén (1997)
Kinetics for hybridization of peptide nucleic acids (PNA) with DNA and RNA studied with the BIAcore technique.Biochemistry, 36 16
J. Straßburger, K. Rejnert (1971)
Diffusion of DNA at very low concentrationsBiopolymers, 10
Sunita Vontel, A. Ramakrishnan, A. Sadana (2000)
An evaluation of hybridization kinetics in biosensors using a single‐fractal analysisBiotechnology and Applied Biochemistry, 31
Vincent Chan, David Graves, Steven McKenzie (1995)
The biophysics of DNA hybridization with immobilized oligonucleotide probes.Biophysical journal, 69 6
Y. Okahata, Masanori Kawase, Kenichi Niikura, F. Ohtake, H. Furusawa, Y. Ebara (1998)
Kinetic measurements of DNA hybridization on an oligonucleotide-immobilized 27-MHz quartz crystal microbalance.Analytical chemistry, 70 7
C. Gadgil, A. Yeckel, J. Derby, Wei-Shou Hu (2004)
A diffusion-reaction model for DNA microarray assays.Journal of biotechnology, 114 1-2
R. Lenigk, Robin Liu, M. Athavale, Zhijian Chen, D. Ganser, Jianing Yang, Cory Rauch, Yingjie Liu, Betty Chan, Huinan Yu, Melissa Ray, Robert Marrero, P. Grodzinski (2002)
Plastic biochannel hybridization devices: a new concept for microfluidic DNA arrays.Analytical biochemistry, 311 1
Min-Kuang Liu, J. Giddings (1993)
Separation and measurement of diffusion coefficients of linear and circular DNAs by flow field-flow fractionationMacromolecules, 26
(1971)
Diffusion of DNA at very low concentration, Biopolymers
(2003)
Modeling of DNA hybridization kinetics for spatially resolved biochips, 317
J. Torre, S. Navarro, M. Martínez (1994)
Hydrodynamic properties of a double-helical model for DNA.Biophysical journal, 66 5
Avraham Halperin, Arnaud Buhot, E. Zhulina (2006)
On the hybridization isotherms of DNA microarrays: the Langmuir model and its extensionsJournal of Physics: Condensed Matter, 18
U. Christensen, N. Jacobsen, V. Rajwanshi, J. Wengel, T. Koch (2001)
Stopped-flow kinetics of locked nucleic acid (LNA)-oligonucleotide duplex formation: studies of LNA-DNA and DNA-DNA interactions.The Biochemical journal, 354 Pt 3
Ying Zhang, D. Hammer, D. Graves (2005)
Competitive hybridization kinetics reveals unexpected behavior patterns.Biophysical journal, 89 5
M. Ferrari, V. Bloomfield (1992)
Scattering and diffusion of mononucleosomal DNA: effects of counterion valence and salt, and DNA concentrationMacromolecules, 25
J. Vanderhoeven, K. Pappaert, B. Dutta, P. Hummelen, G. Desmet (2005)
DNA microarray enhancement using a continuously and discontinuously rotating microchamber.Analytical chemistry, 77 14
D. Gosnell, B. Zimm (1993)
Measurement of diffusion coefficients of DNA in agarose gelMacromolecules, 26
Avraham Halperin, Arnaud Buhot, E. Zhulina (2005)
Brush effects on DNA chips: thermodynamics, kinetics, and design guidelines.Biophysical journal, 89 2
(2003)
Modeling of DNA hybridization kinetics
S. Waidyanatha, Nathaniel Rothman, Guilan Li, Martyn Smith, S. Yin, Stephen Rappaport (2004)
Rapid determination of six urinary benzene metabolites in occupationally exposed and unexposed subjects.Analytical biochemistry, 327 2
M. Heule, A. Manz (2004)
Sequential DNA hybridisation assays by fast micromixing.Lab on a chip, 4 5
K. Pappaert, P. Hummelen, J. Vanderhoeven, G. Baron, G. Desmet (2003)
Diffusion–reaction modelling of DNA hybridization kinetics on biochipsChemical Engineering Science, 58
(1992)
Scattering and diffusion of mononucleosomal
We use the finite element method (Comsol Multiphysics software) to study the kinetics of DNA hybridization and the potential limitation of the reaction rate caused by diffusion effects. The value of the parameters influencing the kinetics can sometimes span several orders of magnitude. Therefore, a wide range of values was used in order to cover the conditions seen in DNA microarrays. In the cases where hybridization is diffusion limited, a convective transport is added to successfully overcome this limitation.
Chemical Engineering Communications – Taylor & Francis
Published: Nov 21, 2007
Keywords: DNA hybridization; Diffusion; Diffusion limitation; Hybridization kinetics
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.