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ENHANCEMENT OF DNA HYBRIDIZATION KINETICS IN MICROARRAYS BY CONVECTIVE TRANSPORT

We use the finite element method (Comsol Multiphysics software) to study the kinetics of DNA hybridization and the potential limitation of the reaction rate caused by diffusion effects. The value of the parameters influencing the kinetics can sometimes span several orders of magnitude. Therefore, a wide range of values was used in order to cover the conditions seen in DNA microarrays. In the cases where hybridization is diffusion limited, a convective transport is added to successfully overcome this limitation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chemical Engineering Communications Taylor & Francis

ENHANCEMENT OF DNA HYBRIDIZATION KINETICS IN MICROARRAYS BY CONVECTIVE TRANSPORT

Abstract

We use the finite element method (Comsol Multiphysics software) to study the kinetics of DNA hybridization and the potential limitation of the reaction rate caused by diffusion effects. The value of the parameters influencing the kinetics can sometimes span several orders of magnitude. Therefore, a wide range of values was used in order to cover the conditions seen in DNA microarrays. In the cases where hybridization is diffusion limited, a convective transport is added to successfully overcome this limitation.
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