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Determination of Tanshinones in Salvia miltiorrhiza Bunge by High-Performance Liquid Chromatography with a Coulometric Electrode Array System

Determination of Tanshinones in Salvia miltiorrhiza Bunge by High-Performance Liquid... Abstract A method was developed for the determination of tanshiones (Cryptotanshinone, tanshinone I, and tanshinone IIA) in Salvia miltiorrhiza Bunge (S. miltiorrhiza Bunge) using high performance liquid chromatography with the coulometric electrode array system (HPLC-CEAD). The analysis was carried out on a column of Hypersil C18 (250 mm × 4.6 mm, 5 µm) with a mobile phase of sodium acetate (pH 2.8, 50 mM) and acetonitrile in the proportion of 45:55. Detection potentials of four electrodes in series were set at − 600, − 400, 100, and 500 mV, respectively. The pH of the mobile phase and the proportion of acetonitrile were optimized in this work. Calibration curves showed good linearity with correlation coefficients of more than 0.9979, the average recoveries of tanshiones were more than 94%, and the relative standard deviations were less than 5.3%. The HPLC-CEAD method was stable, sensitive, and reproducible for the determination of tanshiones S. miltiorrhiza Bunge. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Letters Taylor & Francis

Determination of Tanshinones in Salvia miltiorrhiza Bunge by High-Performance Liquid Chromatography with a Coulometric Electrode Array System

Analytical Letters , Volume 41 (6): 12 – May 5, 2008
12 pages

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References (22)

Publisher
Taylor & Francis
Copyright
Copyright Taylor & Francis Group, LLC
ISSN
1532-236X
eISSN
0003-2719
DOI
10.1080/00032710801978186
Publisher site
See Article on Publisher Site

Abstract

Abstract A method was developed for the determination of tanshiones (Cryptotanshinone, tanshinone I, and tanshinone IIA) in Salvia miltiorrhiza Bunge (S. miltiorrhiza Bunge) using high performance liquid chromatography with the coulometric electrode array system (HPLC-CEAD). The analysis was carried out on a column of Hypersil C18 (250 mm × 4.6 mm, 5 µm) with a mobile phase of sodium acetate (pH 2.8, 50 mM) and acetonitrile in the proportion of 45:55. Detection potentials of four electrodes in series were set at − 600, − 400, 100, and 500 mV, respectively. The pH of the mobile phase and the proportion of acetonitrile were optimized in this work. Calibration curves showed good linearity with correlation coefficients of more than 0.9979, the average recoveries of tanshiones were more than 94%, and the relative standard deviations were less than 5.3%. The HPLC-CEAD method was stable, sensitive, and reproducible for the determination of tanshiones S. miltiorrhiza Bunge.

Journal

Analytical LettersTaylor & Francis

Published: May 5, 2008

Keywords: Coulometric electrode array; HPLC; Salvia miltiorrhiza Bunge , Tanshiones

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