Wound and ethylene induction of the ACC oxidase melon gene CM-ACO1 occurs via two direct and independent transduction pathways

Wound and ethylene induction of the ACC oxidase melon gene CM-ACO1 occurs via two direct and... The enzyme ACC oxidase catalyses the last step of ethylene biosynthesis in plants. Expression of the melon ACC oxidase gene, CM-ACO1, is rapidly induced (within 10 min) by ethylene treatment or upon wounding in leaves. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), inhibited the accumulation of ethylene-induced CM-ACO1 mRNA transcripts, while wound-induced expression of the gene was not affected. The 5′-untranslated region of the CM-ACO1 gene was fused to the β-glucuronidase (GUS) reporter gene and the corresponding transgenic tobacco plants were analysed. Two separate regions of the CM-ACO1 promoter activated GUS expression in response to ethylene treatment and wounding. These results suggest that induction of CM-ACO1 gene expression occurs via two separate signal transduction pathways in response to wounding and ethylene treatment. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Wound and ethylene induction of the ACC oxidase melon gene CM-ACO1 occurs via two direct and independent transduction pathways

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1997 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005902226054
Publisher site
See Article on Publisher Site

Abstract

The enzyme ACC oxidase catalyses the last step of ethylene biosynthesis in plants. Expression of the melon ACC oxidase gene, CM-ACO1, is rapidly induced (within 10 min) by ethylene treatment or upon wounding in leaves. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), inhibited the accumulation of ethylene-induced CM-ACO1 mRNA transcripts, while wound-induced expression of the gene was not affected. The 5′-untranslated region of the CM-ACO1 gene was fused to the β-glucuronidase (GUS) reporter gene and the corresponding transgenic tobacco plants were analysed. Two separate regions of the CM-ACO1 promoter activated GUS expression in response to ethylene treatment and wounding. These results suggest that induction of CM-ACO1 gene expression occurs via two separate signal transduction pathways in response to wounding and ethylene treatment.

Journal

Plant Molecular BiologySpringer Journals

Published: Sep 30, 2004

References

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