Wide-targeted gene expression infers tissue-specific molecular signatures of lipid metabolism in fed and fasted fish

Wide-targeted gene expression infers tissue-specific molecular signatures of lipid metabolism in... European sea bass (Dicentrarchus labrax) is one of the most important species for Mediterranean aquaculture. It is therefore crucial to establish appropriate feeding management regimens and to gain better knowledge of nutritional requirements for this species, exploring not only new feed ingredients and feeding strategies, but also understanding the molecular mechanisms that regulate the metabolism of nutrients. Accordingly, transcriptomic analysis represents a useful nutrigenomic discovery tool for identifying the molecular basis of biological responses to nutrition as well as nutritional biomarkers in fish. This study evaluated how the transcriptional activity of genes controlling lipid metabolism in European sea bass were modulated in a tissue-specific manner in response to fasting and refeeding. Such approach focused on a panel of 29 genes in which desaturases, elongases, triacylglycerol lipases, fatty acid-binding proteins, β-oxidation and oxidative phosphorylation enzymes, phospholipid-related enzymes, and transcription factors that regulate lipid homeostasis were represented. Fasting activated the lipolytic machinery in adipose tissue, liver and muscle of European sea bass, whereas markers of lipogenesis were downregulated in liver and adipose tissue. Genes involved in phospholipid and oxidative metabolism were differentially regulated in liver and skeletal muscle of fasted European sea bass. However, 12 days of refeeding were sufficient, for the most part, to reverse the expression of key genes. Overall, our data clearly showed a tissue-specific regulation of lipid-related genes according to the different metabolic capabilities of each tissue, being the brain the most refractory organ to changes in nutrient and energy availability and liver the most responsive tissue. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Reviews in Fish Biology and Fisheries Springer Journals

Wide-targeted gene expression infers tissue-specific molecular signatures of lipid metabolism in fed and fasted fish

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Springer International Publishing Switzerland
Subject
Life Sciences; Freshwater & Marine Ecology; Zoology
ISSN
0960-3166
eISSN
1573-5184
D.O.I.
10.1007/s11160-015-9408-8
Publisher site
See Article on Publisher Site

Abstract

European sea bass (Dicentrarchus labrax) is one of the most important species for Mediterranean aquaculture. It is therefore crucial to establish appropriate feeding management regimens and to gain better knowledge of nutritional requirements for this species, exploring not only new feed ingredients and feeding strategies, but also understanding the molecular mechanisms that regulate the metabolism of nutrients. Accordingly, transcriptomic analysis represents a useful nutrigenomic discovery tool for identifying the molecular basis of biological responses to nutrition as well as nutritional biomarkers in fish. This study evaluated how the transcriptional activity of genes controlling lipid metabolism in European sea bass were modulated in a tissue-specific manner in response to fasting and refeeding. Such approach focused on a panel of 29 genes in which desaturases, elongases, triacylglycerol lipases, fatty acid-binding proteins, β-oxidation and oxidative phosphorylation enzymes, phospholipid-related enzymes, and transcription factors that regulate lipid homeostasis were represented. Fasting activated the lipolytic machinery in adipose tissue, liver and muscle of European sea bass, whereas markers of lipogenesis were downregulated in liver and adipose tissue. Genes involved in phospholipid and oxidative metabolism were differentially regulated in liver and skeletal muscle of fasted European sea bass. However, 12 days of refeeding were sufficient, for the most part, to reverse the expression of key genes. Overall, our data clearly showed a tissue-specific regulation of lipid-related genes according to the different metabolic capabilities of each tissue, being the brain the most refractory organ to changes in nutrient and energy availability and liver the most responsive tissue.

Journal

Reviews in Fish Biology and FisheriesSpringer Journals

Published: Nov 14, 2015

References

  • Zebrafish cDNA encoding multifunctional fatty acid elongase involved in production of eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids
    Agaba, M; Tocher, DR; Dickson, CA; Dick, JR; Teale, AJ
  • Starvation response in mouse liver shows strong correlation with life-span-prolonging processes
    Bauer, M; Hamm, AC; Bonaus, M; Jacob, A; Jaekel, J; Schorle, H; Pankratz and, MJ; Katzenberger, JD
  • Wide-gene expression analysis of lipid-relevant genes in nutritionally challenged gilthead sea bream (Sparus aurata)
    Benedito-Palos, L; Ballester-Lozano, GF; Pérez-Sánchez, J
  • Growth and feed utilization of gilthead sea bream (Sparus aurata, L.) fed to satiation and restrictively at increasing dietary energy levels
    Bonaldo, A; Isani, G; Fontanillas, R; Parma, L; Grilli, E; Gatta, PP

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