1021-4437/01/4803- $25.00 © 2001
Russian Journal of Plant Physiology, Vol. 48, No. 3, 2001, pp. 377–381. Translated from Fiziologiya Rastenii, Vol. 48, No. 3, 2001, pp. 441–446.
Original Russian Text Copyright © 2001 by Salina, Leonova, Röder, Laikova, Maystrenko, Budashkina, Shumnyi.
Microsatellites, or tandem DNA sequences with
repeats that do not exceed 6 bp, were found in all
eukaryotic genomes. The studies of microsatellite
regions led to the development of a new class of poly-
morphic markers, that is, microsatellite markers, which
are widely used for mapping eukaryote genomes .
The basic characteristics of these markers comprise the
–High information content—the markers are
codominant, with different alleles in closely related
–PCR analysis of these markers is easy and does not
involve radioactive materials.
–The sequences for all microsatellite primers are
available, and there is no need to exchange the samples
–The method requires a small quantity of DNA from
any plant tissues.
–The microsatellites are abundant (with the fre-
quency of 1/10
bp of genome).
The development of microsatellite markers is a
laborious work. It includes such steps as searching for
the microsatellite regions in the genomic libraries of
particular plant species with synthetic probes, sequenc-
ing selected DNA fragments, designing primers to the
microsatellite regions, and verifying a particular micro-
satellite sequence as the efﬁcient molecular marker. As
a whole, to obtain one microsatellite marker, one must
screen about 3000 clones from a grass genomic library.
The analysis of DNA fragments ampliﬁed with the
primers recognizing the microsatellite regions is rather
simple. It usually employs PAGE under native and
denaturing conditions [2, 3]. In the latter case, nonra-
dioactive methods of visualization, such as using ﬂuo-
rescein-labeled primers  and gel silver staining ,
are most popular.
Recently, over 800 microsatellite markers were
added to the molecular genetic map of common wheat
= 42) . These markers can
be used to map genes and analyze wheat hybrid
The goal of the current study was to assess the efﬁ-
ciency of the microsatellite markers for analyzing
reconstructed wheat genomes and mapping genes. We
carried out the microsatellite analysis of the introgres-
sive lines of common wheat bred by crossing several
Wheat Microsatellites: The Prospects of Application for Gene
Mapping and Analysis of the Reconstructed Genomes
E. A. Salina*, I. N. Leonova*, M. S. Röder**, L. I. Laikova*, O. I. Maystrenko
E. B. Budashkina*, and V. K. Shumnyi*
* Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences,
pr. Akademika Lavrent’eva 10, Novosibirsk, 630090 Russia;
fax: 7 (3832) 33-1278; e-mail: email@example.com
** Institute of Plant Genetic and Crop Research, Gatersleben, Germany
Received March 16, 2000
were used to map the
genes of the induced
sphaerococcoid mutants of
L. and to analyze the introgressive lines of common wheat,
obtained by crossing several common wheat cultivars to
Zhuk.; these lines carry the
resistance to leaf rust. All sphaerococcoid genes were linked to centromeric markers of the short and long arms
of chromosomes of homoeologous group 3 of
locus was located between the markers
; and the
was located between
. The introgressive lines of common wheat carry the following substitutions from
most of 2A and 2B and telomeric region of the 5AL chromosome in the line 821, the same introgression and
also the completely substituted chromosome 4B in line 837, and the partially substituted chromosomes 2A and
2B in line 842. The introgression of the genomic material from
into the chromosomes of
homoeologous group 2 was the common trait of all three lines resistant to leaf rust. The authors discuss the fea-
sibility of using microsatellite-derived data for analyzing nonmapped wheat species, linking new genes to
wheat molecular genetic maps, and analyzing wheat genomes of diverse hybrid origins.
Key words: Triticum aestivum - Triticum timopheevii - microsatellites - mapping S1, S2, and S3 genes - Lr genes
: PCR—polymerase chain reaction.