Voltage-dependent Closing of Porin Channels: Analysis of Relaxation Kinetics

Voltage-dependent Closing of Porin Channels: Analysis of Relaxation Kinetics The anion-selective porin Omp34 from Acidovorax delafieldii was unidirectionally reconstituted in planar lipid membranes. Pore closing was recorded particularly at low salt conditions for negative and positive membrane potentials in the range of ±10 to ±100 mV. Relaxation curves were fitted by exponential functions in order to describe and to analyze the voltage-dependent behavior. Omp34 exhibited the following characteristics: (i) The channels are asymmetric with respect to closing characteristics and corresponding functional parameters. (ii) Relaxation curves can be fitted by a single exponential function in the low voltage range only, at ≥40 mV combinations of two exponential functions are required. (iii) Beyond 60 to 70 mV a third exponential function is necessary to fit the fast closing components properly. The time constants differ by two to three orders of magnitude. (iv) Hysteresis in I-V-diagrams originate from slow relaxation components which are different for positive and negative voltages. The implications for models aiming at description of voltage-dependent closing are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Voltage-dependent Closing of Porin Channels: Analysis of Relaxation Kinetics

Loading next page...
Copyright © Inc. by 1998 Springer-Verlag New York
Life Sciences; Biochemistry, general; Human Physiology
Publisher site
See Article on Publisher Site

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial