Bombardment of a highly expressed dicot tRNAala(GAC) gene into Zea mays bz-E2 or bz-E5 coleoptiles causes suppression of an Ala458 →Val missense mutation, visualized by the development of anthocyanin pigment. Missense suppression is blocked by mutation of tRNAala(GAC) at a site that prevents aminoacylation by the dicot alanyl-tRNA synthetase, indicating that features identified for expression and utilization of dicot tRNAs also function in monocots. This assay of the expression and utilization of tRNAala(GAC) also can be used to study a variety of tRNAs and their genes, most of which can be relatively easily altered to be charged by alanyl tRNA synthetase.
Plant Molecular Biology – Springer Journals
Published: Mar 22, 2006
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