Visualizing bz1 missense suppression in Zea mays: an assay for monocot tRNA expression and utilization

Visualizing bz1 missense suppression in Zea mays: an assay for monocot tRNA expression and... Bombardment of a highly expressed dicot tRNAala(GAC) gene into Zea mays bz-E2 or bz-E5 coleoptiles causes suppression of an Ala458 →Val missense mutation, visualized by the development of anthocyanin pigment. Missense suppression is blocked by mutation of tRNAala(GAC) at a site that prevents aminoacylation by the dicot alanyl-tRNA synthetase, indicating that features identified for expression and utilization of dicot tRNAs also function in monocots. This assay of the expression and utilization of tRNAala(GAC) also can be used to study a variety of tRNAs and their genes, most of which can be relatively easily altered to be charged by alanyl tRNA synthetase. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Visualizing bz1 missense suppression in Zea mays: an assay for monocot tRNA expression and utilization

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2006 by Springer Science+Business Media B.V.
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-006-0050-7
Publisher site
See Article on Publisher Site

Abstract

Bombardment of a highly expressed dicot tRNAala(GAC) gene into Zea mays bz-E2 or bz-E5 coleoptiles causes suppression of an Ala458 →Val missense mutation, visualized by the development of anthocyanin pigment. Missense suppression is blocked by mutation of tRNAala(GAC) at a site that prevents aminoacylation by the dicot alanyl-tRNA synthetase, indicating that features identified for expression and utilization of dicot tRNAs also function in monocots. This assay of the expression and utilization of tRNAala(GAC) also can be used to study a variety of tRNAs and their genes, most of which can be relatively easily altered to be charged by alanyl tRNA synthetase.

Journal

Plant Molecular BiologySpringer Journals

Published: Mar 22, 2006

References

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