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Viral metagenomics analysis and eight novel viral genomes identified from the Dushanzi mud volcanic soil in Xinjiang, China

Viral metagenomics analysis and eight novel viral genomes identified from the Dushanzi mud... Purpose Viruses are the most abundant biological entities and play significant biological roles in marine system. However, little is known about their biodiversity in mud volcanic soil, which is salty and covered with emitting sludge. In this study, we report metagenomic analysis results of viral community composition and function from the Dushanzi mud volcanic soil (named NHS) in Xinjiang, China, and provide a comparative analysis with other environmental types. Materials and methods We utilized metagenomes to obtain the virus data and carried out viral automatic phylogenetic and functional analyses by the metagenomics analysis server. After assembling by metaSPAdes, scaffolds were rapidly identified and annotated with the PHAge Search Tool in order to identify new phage genomes. Results and discussion For the ssDNA viral group, Microviridae were the most abundant viral family, which were above 95%. Other plant viruses (Geminiviridae, Nanoviridae) and animal viruses (Circoviridae, Parvoviridae) were also detected. The majority of viral significant hits belonged to Siphoviridae (80.36%). Phages, which infect bacteria, were widely distributed in the soil sample. There were eight novel viral genomes identified in the NHS virome. The two longest scaffolds were annotated to Pseudomonas aeruginosa phage YMC11 and Acinetobacter baumannii phage LZ35, respectively. Other six http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Soils and Sediments Springer Journals

Viral metagenomics analysis and eight novel viral genomes identified from the Dushanzi mud volcanic soil in Xinjiang, China

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Environment; Soil Science & Conservation; Environment, general; Environmental Physics
ISSN
1439-0108
eISSN
1614-7480
DOI
10.1007/s11368-018-2045-9
Publisher site
See Article on Publisher Site

Abstract

Purpose Viruses are the most abundant biological entities and play significant biological roles in marine system. However, little is known about their biodiversity in mud volcanic soil, which is salty and covered with emitting sludge. In this study, we report metagenomic analysis results of viral community composition and function from the Dushanzi mud volcanic soil (named NHS) in Xinjiang, China, and provide a comparative analysis with other environmental types. Materials and methods We utilized metagenomes to obtain the virus data and carried out viral automatic phylogenetic and functional analyses by the metagenomics analysis server. After assembling by metaSPAdes, scaffolds were rapidly identified and annotated with the PHAge Search Tool in order to identify new phage genomes. Results and discussion For the ssDNA viral group, Microviridae were the most abundant viral family, which were above 95%. Other plant viruses (Geminiviridae, Nanoviridae) and animal viruses (Circoviridae, Parvoviridae) were also detected. The majority of viral significant hits belonged to Siphoviridae (80.36%). Phages, which infect bacteria, were widely distributed in the soil sample. There were eight novel viral genomes identified in the NHS virome. The two longest scaffolds were annotated to Pseudomonas aeruginosa phage YMC11 and Acinetobacter baumannii phage LZ35, respectively. Other six

Journal

Journal of Soils and SedimentsSpringer Journals

Published: Jun 5, 2018

References