ISSN 10227954, Russian Journal of Genetics, 2012, Vol. 48, No. 8, pp. 819–822. © Pleiades Publishing, Inc., 2012.
Original Russian Text © N.V. Kovalyuk, V.F. Satsuk, A.E. Volchenko, 2012, published in Genetika, 2012, Vol. 48, No. 8, pp. 962–965.
major histocompatibility com
plex gene has highly polymorphic regions [1, 2], thus
being suitable for population studies and, in particular,
heterozygosity analysis in cattle groups. Functionally,
gene determines the level of the pri
mary immune response to viral and bacterial patho
gens [3–5]. There is also experimental evidence that
genotype is associated with milk pro
ductivity in cattle [6–9].
MATERIALS AND METHODS
Molecular biological methods were used to exam
ine 263 sperm samples of Holstein stud bulls involved
in the artificial insemination program of Krasnodar
krai (the bulls were from the breeding facilities GTsV
and Moskovskoe) and 650 blood samples of Holstein
cows and heifers (farms Kuban’ (Ust’Labinskii raion,
= 500), Rossiya (Krasnoarmeiskii raion,
and Khutorok (Novokubanskii raion,
= 50)). DNA
was isolated from sperm and blood specimens with the
help of a Diatom Prep 100 kit (Izogen) and was used
for the polymerase chain reaction (PCR) with primers
HLO30 and HLO32 (a PCR product of 284 bp) .
Restriction fragment length polymorphism (RFLP)
analysis was carried out with
We observed that six or seven RFLP patterns (out of
more than 50 possible ones) predominated in our sam
ple of Holstein cows and heifers (
= 650) (Table 1),
accounting for more than 75% of the sample.
A similar distribution of RFLP patterns was
observed in samples of cows and heifers of Russian and
foreign origins, as well as in samples of stud bulls from
various Russian breeding facilities (Table 2).
Variation of the
Gene in Dairy Cattle and Its Effect
on the Viability Parameters
N. V. Kovalyuk
, V. F. Satsuk
, and A. E. Volchenko
NorthCaucasian Institute of Animal Breeding, Russian Academy of Agricultural Sciences, Krasnodar, 350055 Russia
ASTER ResearchandProduction Agricultural Enterprise, Krasnodar, 350055 Russia
Received October 12, 2011
—Genotyping of the
alleles was performed in dairy cattle of Krasnodar krai and Hol
stein stud bulls. Loss of heterozygosity, which decreased the reproductive parameters, was observed. It was
proposed that stud bulls be selected on the basis of their genotyping at the
gene to prevent further
decay of cattle viability.
Allele frequencies of the
locus in blackandwhite Holstein cows and heifers
3 8 11 16 22 23 24
Kuban’, Ust’Labinskii raion (
= 500) – 7 5 19 19 11 18 21
Rossiya, Krasnoarmeiskii raion (
= 100) 11 16 10 19 9 9 14 12
= 50) 11 7 17 10 8 4 20 23
Note: * Ordinal numbers of RFLP patterns are indicated.