Using adaptive mutagenesis system for identification of early genes encoding de novo purine biosynthesis in methylotrophic yeast Pichia methanolica MH4

Using adaptive mutagenesis system for identification of early genes encoding de novo purine... A collection of spontaneous “Roman’s mutants” (1654 mutants) for early genes of purine biosynthesis PUR1–PUR5 was obtained from 16 parental ade1 (pur6) and ade2(pur7) strains of the methylotrophic yeast Pichia methanolica. Two genes, bifunctiional ADE7,4(PUR2,5) and ADE5(PUR4), were identified earlier. For identification of the two remaining early genes (ADE3 and ADE8), a novel approach was used: a comparison of spectra of spontaneous Roman’s mutants and relative sizes of genes (with regard to the length of polypeptides in amino acid residues). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Using adaptive mutagenesis system for identification of early genes encoding de novo purine biosynthesis in methylotrophic yeast Pichia methanolica MH4

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2009 by Pleiades Publishing, Ltd.
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S102279540910007X
Publisher site
See Article on Publisher Site

Abstract

A collection of spontaneous “Roman’s mutants” (1654 mutants) for early genes of purine biosynthesis PUR1–PUR5 was obtained from 16 parental ade1 (pur6) and ade2(pur7) strains of the methylotrophic yeast Pichia methanolica. Two genes, bifunctiional ADE7,4(PUR2,5) and ADE5(PUR4), were identified earlier. For identification of the two remaining early genes (ADE3 and ADE8), a novel approach was used: a comparison of spectra of spontaneous Roman’s mutants and relative sizes of genes (with regard to the length of polypeptides in amino acid residues).

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 11, 2009

References

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