Russian Journal of Applied Chemistry, 2012, Vol. 85, No. 1, pp. 136−139.
Pleiades Publishing, Ltd., 2012.
Original Russian Text © R.I. Yusupova, A.I. Kurmaeva, M.V. Potapova, E.M. Kulagina, V.P. Barabanov, 2012, published in Zhurnal Prikladnoi Khimii, 2012,
Vol. 85, No. 1, pp. 142−146.
AND POLYMERIC MATERIALS
Use of Polyelectrolytes for Puriﬁ cation
of Crude Ribonucleic Acid
R. I. Yusupova, A. I. Kurmaeva, M. V. Potapova, E. M. Kulagina, and V. P. Barabanov
Kazan State University of Technology, Kazan, Tatarstan, Russia
Received April 12, 2011
Abstract—The effect of polymeric reagents on a ribonucleic acid suspension was studied. The polyelectrolytic
complex based on polyacrylic acid proved to be an effective ﬂ occulant for colloidal impurities in crude ribonucleic
acid. The efﬁ ciency of ribonucleic acid puriﬁ cation was evaluated.
In complex processing of biomass, it is appropriate
to recover as target products nucleic acids and proteins,
which are the most valuable components of yeasts from
the viewpoint of biological activity. It is known [1, 2]
that a yeast cell consists of the following components,
%: protein 50, carbohydrates 20, nucleic acids 5–10,
with the remainder being lipids and other substances.
However, in the existing processes nucleic components
are not recovered separately and are not utilized. At the
same time, it is well known that the absence of nucleic
acids in protein-containing products decreases their
The goal of this study is purification of crude
ribonucleic acid (RNA). The complex processing of the
yeast biomass involves two steps: (1) recovery of RNA
from the yeast biomass, i.e., preparation of denucleized
biomass, and (2) processing of the denucleized biomass
into protein-containing products.
Various yeast cultures are used as raw materials for
preparing RNA, including Saccharomyces cerevisiae
baker’s yeast and Candida genus forage yeasts grown
on various substrates (Paradiene hydrocarbons, sulﬁ te
liquor, alcohol, molasses). In this study we chose as raw
material Saccharomyces cerevisiae culture. The raw
material was in the form of yeast suspension (1), yeast
paste (thickened cell biomass, (2), and yeast concentrate
prepared by drying yeast paste (3). Crude RNA can be
recovered from these raw materials under laboratory
conditions (see scheme).
Scheme of recovery of ribonucleic acid from yeast biomass
Crude ribonucleic acid
Drying at 25°C