A viral vector that expressed foreign genes was engineered using a cDNA clone of broad bean mottle bromovirus (BBMV) defective interfering (DI) RNA. The expression vector required an active ORF to ensure efficient accumulation and replication in the host plants. After mechanical inoculation with BBMV RNAs, expression of the green fluorescent protein (GFP) reporter was driven by DI RNA constructs during consecutive passages through broad bean plants. Our data prove that BBMV DI RNAs, whose yield is similar to the virus genomic components, are useful as gene vectors after mechanical inoculation on legume plants.
Archives of Virology – Springer Journals
Published: Sep 1, 2008
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera